Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s11356-023-28144-1
Lizenz creative commons licence
Titel (primär) Proteogenomics of the novel Dehalobacterium formicoaceticum strain EZ94 highlights a key role of methyltransferases during anaerobic dichloromethane degradation
Autor Wasmund, K.; Trueba-Santiso, A.; Vicent, T.; Adrian, L.; Vuilleumier, S.; Marco-Urrea, E.
Quelle Environmental Science and Pollution Research
Erscheinungsjahr 2023
Department UBT
Band/Volume 30
Heft 33
Seite von 80602
Seite bis 80612
Sprache englisch
Topic T7 Bioeconomy
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs11356-023-28144-1/MediaObjects/11356_2023_28144_MOESM1_ESM.xlsx
Keywords Dehalobacterium; Anaerobic dichloromethane degradation; Methyltransferases; Wood-Ljungdahl pathway; Shotgun proteomics
Abstract Dichloromethane (DCM, methylene chloride) is a toxic, high-volume industrial pollutant of long-standing. Anaerobic biodegradation is crucial for its removal from contaminated environments, yet prevailing mechanisms remain unresolved, especially concerning dehalogenation. In this study, we obtained an assembled genome of a novel DCM-degrading strain, Dehalobacterium formicoaceticum strain EZ94, from a stable DCM-degrading consortium, and we analyzed its proteome during degradation of DCM. A gene cluster recently predicted to play a major role in anaerobic DCM catabolism (the mec cassette) was found. Methyltransferases and other proteins encoded by the mec cassette were among the most abundant proteins produced, suggesting their involvement in DCM catabolism. Reductive dehalogenases were not detected. Genes and corresponding proteins for a complete Wood-Ljungdahl pathway, which could enable further metabolism of DCM carbon, were also found. Unlike for the anaerobic DCM degrader “Ca. F. warabiya,” no genes for metabolism of the quaternary amines choline and glycine betaine were identified. This work provides independent and supporting evidence that mec-associated methyltransferases are key to anaerobic DCM metabolism.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27345
Wasmund, K., Trueba-Santiso, A., Vicent, T., Adrian, L., Vuilleumier, S., Marco-Urrea, E. (2023):
Proteogenomics of the novel Dehalobacterium formicoaceticum strain EZ94 highlights a key role of methyltransferases during anaerobic dichloromethane degradation
Environ. Sci. Pollut. Res. 30 (33), 80602 - 80612 10.1007/s11356-023-28144-1