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Referenztyp Zeitschriften
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Creative Commons Lizenz creative commons licence
Titel (primär) IL17F expression as an early sign of oxidative stress-induced cytotoxicity/apoptosis
Autor Bauer, M.; Fink, B.; Anderegg, U.; Röder, S.; Zenclussen, A.C.
Journal / Serie Genes
Erscheinungsjahr 2022
Department IMMU
Band/Volume 13
Heft 10
Seite von art. 1739
Sprache englisch
Topic T9 Healthy Planet
Supplements https://www.mdpi.com/article/10.3390/genes13101739/s1
Keywords L17F; apoptosis; BEAS-2B; THP-1; fibrosis; primary dermal fibroblasts
Abstract Interleukin 17F (IL17F) has been found to be involved in various inflammatory pathologies and has recently become a target for therapeutic purposes. In contrast to IL17F secreted by immune cells, the focus of this study is to describe the triggers of IL17F release in non-immune cells with a particular focus on IL17F-induced fibrosis. IL17F induction was examined in human lung epithelial (BEAS-2B) and myeloid cell lines as well as in peripheral blood mononuclear cells after in vitro exposure to aqueous cigarette smoke extract (CSE), inorganic mercury, cadmium or the apoptosis inducer brefeldin A. Fibrosis was examined in vitro, evaluating the transition of human primary dermal fibroblasts to myofibroblasts. We observed that all stressors were able to induce IL17F gene expression regardless of cell type. Interestingly, its induction was associated with cytotoxic/apoptotic signs. Inhibiting oxidative stress by N-acetylcysteine abrogated CSE-induced cytotoxic and IL17F-inducing effects. The induction of IL17F was accompanied by IL17F protein expression. The transition of fibroblasts into myofibroblasts was not influenced by either recombinant IL17F or supernatants of CSE-exposed BEAS-2B. In addition to IL17F secretion by specialized or activated immune cells, we underscored the cell type-independent induction of IL17F by mechanisms of inhibitable oxidative stress-induced cytotoxicity. However, IL17F was not involved in dermal fibrosis under the conditions used in this study.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26623
Bauer, M., Fink, B., Anderegg, U., Röder, S., Zenclussen, A.C. (2022):
IL17F expression as an early sign of oxidative stress-induced cytotoxicity/apoptosis
Genes 13 (10), art. 1739