Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.envadv.2022.100284
Lizenz creative commons licence
Titel (primär) Rapid determination of serum albumin partition coefficients using affinity chromatography
Autor Ulrich, N.; Böhme, A.
Quelle Environmental Advances
Erscheinungsjahr 2022
Department OEC
Band/Volume 9
Seite von art. 100284
Sprache englisch
Topic T9 Healthy Planet
Keywords Serum albumin partition coefficient; Affinity chromatography; Linear solvation; energy relationship; LSER

Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target side are derived from nominal concentrations. Sorption to serum albumin is often characterized by the serum albumin-water partition coefficient KSA/W, which is traditionally determined using dialysis experiments. However, dialysis approaches are time-consuming and require a large amount of pure protein. Thus, we investigated affinity chromatography as a fast alternative to characterize the sorption of chemicals to serum albumin. To this end, the application of two types of serum albumin columns (bovine vs. human), different temperature settings (30°C vs. 37°C), and the impact of 2-propanol as modifier in the mobile phase were evaluated. Moreover, a linear regression model was calibrated with 49 experimentally determined log KSA/W values from the literature to predict sorption to serum albumin directly from the retention factor log kHSA.

In addition, we tested a prediction approach based on linear solvation energy relationships and compared the two procedures. Further, we compiled a dataset of log kHSA values for 378 chemicals, which we classified according to their ionization state and structure, and according to environmentally relevant classes like persistent, mobile, and toxic chemicals, pesticides, and plastic additives. There was no specific trend observed for particular classes of chemicals, which means that there is a demand for determining the sorption to serum albumin for these chemicals for the correction of the effect concentration in bioassays, e.g., and to assess bioaccumulation in higher tier organisms such as fish or rodents.

dauerhafte UFZ-Verlinkung
Ulrich, N., Böhme, A. (2022):
Rapid determination of serum albumin partition coefficients using affinity chromatography
Environ. Adv. 9 , art. 100284 10.1016/j.envadv.2022.100284