Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/microorganisms10081610
Lizenz creative commons licence
Titel (primär) Adaptation and resistance: How Bacteroides thetaiotaomicron copes with the bisphenol A substitute bisphenol F
Autor Riesbeck, S.; Petruschke, H.; Rolle-Kampczyk, U.; Schori, C.; Ahrens, C.H.; Eberlein, C. ORCID logo ; Heipieper, H.J. ORCID logo ; von Bergen, M.; Jehmlich, N. ORCID logo
Quelle Microorganisms
Erscheinungsjahr 2022
Department UBT; MOLSYB
Band/Volume 10
Heft 8
Seite von art. 1610
Sprache englisch
Topic T9 Healthy Planet
T7 Bioeconomy
Keywords xenobiotics; bisphenols; gut microbiome; fatty acid methyl ester; short-chain fatty acids; proteomics
Abstract Bisphenols are used in the process of polymerization of polycarbonate plastics and epoxy resins. Bisphenols can easily migrate out of plastic products and enter the gastrointestinal system. By increasing colonic inflammation in mice, disrupting the intestinal bacterial community structure and altering the microbial membrane transport system in zebrafish, bisphenols seem to interfere with the gut microbiome. The highly abundant human commensal bacterium Bacteroides thetaiotaomicron was exposed to bisphenols (Bisphenol A (BPA), Bisphenol F (BPF), Bisphenol S (BPS)), to examine the mode of action, in particular of BPF. All chemicals caused a concentration-dependent growth inhibition and the half-maximal effective concentration (EC50) corresponded to their individual logP values, a measure of their hydrophobicity. B. thetaiotaomicron exposed to BPF decreased membrane fluidity with increasing BPF concentrations. Physiological changes including an increase of acetate concentrations were observed. On the proteome level, a higher abundance of several ATP synthase subunits and multidrug efflux pumps suggested an increased energy demand for adaptive mechanisms after BPF exposure. Defense mechanisms were also implicated by a pathway analysis that identified a higher abundance of members of resistance pathways/strategies to cope with xenobiotics (i.e., antibiotics). Here, we present further insights into the mode of action of bisphenols in a human commensal gut bacterium regarding growth inhibition, and the physiological and functional state of the cell. These results, combined with microbiota-directed effects, could lead to a better understanding of host health disturbances and disease development based on xenobiotic uptake.
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Riesbeck, S., Petruschke, H., Rolle-Kampczyk, U., Schori, C., Ahrens, C.H., Eberlein, C., Heipieper, H.J., von Bergen, M., Jehmlich, N. (2022):
Adaptation and resistance: How Bacteroides thetaiotaomicron copes with the bisphenol A substitute bisphenol F
Microorganisms 10 (8), art. 1610 10.3390/microorganisms10081610