Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.bbrc.2006.03.135
Titel (primär) Interactions of recombinant prions with compounds of therapeutical significance
Autor Georgieva, D.; Schwark, D.; von Bergen, M.; Redecke, L.; Genov, N.; Betzel, C.
Quelle Biochemical and Biophysical Research Communications
Erscheinungsjahr 2006
Department PROTEOM
Band/Volume 344
Heft 2
Seite von 463
Seite bis 470
Sprache englisch
Keywords Prion protein; Proteinase; Proteolysis; Protein misfolding; Dynamic light scattering; Structural transformation
Abstract The transformation of the cellular prion protein (PrPC) into the infectious form (PrPSc) is implicated in the invariably fatal transmissible spongiform encephalopathies. To identify a mechanism to prevent the undesired PrPC ? PrPSc transformation, we investigated the interactions of recombinant prion proteins with a number of potential therapeutic agents which inhibit the PrPSc formation, infectivity, and the accumulation of the misfolded form. We show that the prion aggregates formed in the presence of six compounds have no ß-structure, which is typical of the infectious form, and possess considerably higher a-helical content than the normal PrPC. The investigated compounds stimulate the formation of a-helices and the destruction of ß-structure. They prevent the transformation of a-helical structure into ß-sheets. Probably, this is the reason for the resistance to PrPC ? PrPSc transformation in the presence of these compounds. The results may be useful for the future therapy of neurodegenerative diseases.
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Georgieva, D., Schwark, D., von Bergen, M., Redecke, L., Genov, N., Betzel, C. (2006):
Interactions of recombinant prions with compounds of therapeutical significance
Biochem. Biophys. Res. Commun. 344 (2), 463 - 470 10.1016/j.bbrc.2006.03.135