Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/metabo12070583
Lizenz creative commons licence
Titel (primär) Ring trial on quantitative assessment of bile acids reveals a method- and analyte-specific accuracy and reproducibility
Autor Haange, S.-B. ORCID logo ; Till, A.; Bergh, P.-O.; Fauler, G.; Gigl, M.; Löfgren-Sandblom, A.; Schaap, F.G.; Clavel, T.; Trautwein, C.; Fenske, W.; Kleigrewe, K.; Marschall, H.-U.; Olde Damink, S.W.M.; Moustafa, T.; von Bergen, M.; Rolle-Kampczyk, U.
Quelle Metabolites
Erscheinungsjahr 2022
Department MOLSYB
Band/Volume 12
Heft 7
Seite von art. 583
Sprache englisch
Topic T9 Healthy Planet
Keywords bile acids; ring trial; LC-MS/MS; absolute quantification; human serum; murine serum
Abstract (1) Background: Bile acids are a key mediator of the molecular microbiome-host interaction, and various mass spectrometry-based assays have been developed in the recent decade to quantify a wide range of bile acids. We compare existing methodologies to harmonize them. (2) Methods: Methodology for absolute quantification of bile acids from six laboratories in Europe were compared for the quantification of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) and conjugated products glycocholic acid (GCA) and taurocholic acid (TCA). For the bacterially modified secondary bile acids, the quantification of deoxycholic acid (DCA) and lithocholic acid (LCA) was compared. For the murine bile acids, we used the primary muricholic acids (α-MCA and, β-MCA) and the intestinally produced secondary bile acid muricholic (ω-MCA). The standards were spiked into methanol:water (1:1) mix as well as in human and murine serum at either low concentration range (150–3000 nM) or high concentration range (1500–40,000 nM). (3) Results: The precision was better for higher concentrations. Measurements for the hydrophobic unconjugated bile acids LCA and ω-MCA were the most challenging. (4) Conclusions: The quality assessments were generally very similar, and the comprehensive analyses demonstrated that data from chosen locations can be used for comparisons between studies.
dauerhafte UFZ-Verlinkung
Haange, S.-B., Till, A., Bergh, P.-O., Fauler, G., Gigl, M., Löfgren-Sandblom, A., Schaap, F.G., Clavel, T., Trautwein, C., Fenske, W., Kleigrewe, K., Marschall, H.-U., Olde Damink, S.W.M., Moustafa, T., von Bergen, M., Rolle-Kampczyk, U. (2022):
Ring trial on quantitative assessment of bile acids reveals a method- and analyte-specific accuracy and reproducibility
Metabolites 12 (7), art. 583 10.3390/metabo12070583