Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1002/cyto.a.24658 |
Lizenz  |
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| Titel (primär) | The potential of multispectral imaging flow cytometry for environmental monitoring |
| Autor | Dunker, S.; Boyd, M.; Durka, W.
; Erler, S.; Harpole, W.S.
; Henning, S.; Herzschuh, U.; Hornick, T.; Knight, T.; Lips, S.; Mäder, P.; Motivans Švara, E.; Mozarowski, S.; Rakosy, D.; Römermann, C.; Schmitt-Jansen, M.; Stoof-Leichsenring, K.; Stratmann, F.; Treudler, R.; Virtanen, R.; Wendt-Potthoff, K.
; Wilhelm, C. |
| Quelle | Cytometry Part A |
| Erscheinungsjahr | 2022 |
| Department | BIOTOX; BZF; SEEFO; iDiv; PHYDIV |
| Band/Volume | 101 |
| Heft | 9 |
| Seite von | 782 |
| Seite bis | 799 |
| Sprache | englisch |
| Topic | T5 Future Landscapes |
| Daten-/Softwarelinks | https://doi.org/10.48758/ufz.12577 |
| Keywords | environmental monitoring; imaging flow cytometry; plant traits |
| Abstract | Environmental monitoring involves the quantification of microscopic cells and parti-cles such as algae, plant cells, pollen, or fungal spores. Traditional methods using con-ventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC)allows measurement of up to 5000 particles per second from a fluid suspension andcan simultaneously capture up to 12 images of every single particle for brightfieldand different spectral ranges, with up to 60x magnification. The high throughput ofMIFC has high potential for increasing the amount and accuracy of environmentalmonitoring, such as for plant-pollinator interactions, fossil samples, air, water or foodquality that currently rely on manual microscopic methods. Automated recognition ofparticles and cells is also possible, when MIFC is combined with deep-learning com-putational techniques. Furthermore, various fluorescence dyes can be used to stainspecific parts of the cell to highlight physiological and chemical features including:vitality of pollen or algae, allergen content of individual pollen, surface chemical com-position (carbohydrate coating) of cells, DNA- or enzyme-activity staining. Here, weoutline the great potential for MIFC in environmental research for a variety ofresearch fields and focal organisms. In addition, we provide best practicerecommendations. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26168 |
| Dunker, S., Boyd, M., Durka, W., Erler, S., Harpole, W.S., Henning, S., Herzschuh, U., Hornick, T., Knight, T., Lips, S., Mäder, P., Motivans Švara, E., Mozarowski, S., Rakosy, D., Römermann, C., Schmitt-Jansen, M., Stoof-Leichsenring, K., Stratmann, F., Treudler, R., Virtanen, R., Wendt-Potthoff, K., Wilhelm, C. (2022): The potential of multispectral imaging flow cytometry for environmental monitoring Cytom. Part A 101 (9), 782 - 799 10.1002/cyto.a.24658 |
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