Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3389/fmicb.2022.872576
Lizenz creative commons licence
Titel (primär) Insight to the molecular mechanisms underpinning the mycoremediation of multiple metals by proteomic technique
Autor Dey, P.; Malik, A.; Singh, D.K.; Haange, S.-B. ORCID logo ; von Bergen, M.; Jehmlich, N. ORCID logo
Quelle Frontiers in Microbiology
Erscheinungsjahr 2022
Department MOLSYB
Band/Volume 13
Seite von art. 872576
Sprache englisch
Topic T9 Healthy Planet
Keywords Multi-metal; Proteomics; Mycoremediation; LC-MS/MS; Fungi
Abstract We investigated the fungus Aspergillus fumigatus PD-18 responses when subjected to the multimetal combination (Total Cr, Cd2+, Cu2+, Ni2+, Pb2+, and Zn2+) in synthetic composite media. To understand how multimetal stress impacts fungal cells at the molecular level, the cellular response of A. fumigatus PD-18 to 30 mg/L multimetal stress (5 mg/L of each heavy metal) was determined by proteomics. The comparative fungal proteomics displayed the remarkable inherent intracellular and extracellular mechanism of metal resistance and tolerance potential of A. fumigatus PD-18. This study reported 2,238 proteins of which 434 proteins were exclusively expressed in multimetal extracts. The most predominant functional class expressed was for cellular processing and signaling. The type of proteins and the number of proteins that were upregulated due to various stress tolerance mechanisms were post-translational modification, protein turnover, and chaperones (42); translation, ribosomal structure, and biogenesis (60); and intracellular trafficking, secretion, and vesicular transport (18). In addition, free radical scavenging antioxidant proteins, such as superoxide dismutase, were upregulated upto 3.45-fold and transporter systems, such as protein transport (SEC31), upto 3.31-fold to combat the oxidative stress caused by the multiple metals. Also, protein–protein interaction network analysis revealed that cytochrome c oxidase and 60S ribosomal protein played key roles to detoxify the multimetal. To the best of our knowledge, this study of A. fumigatus PD-18 provides valuable insights toward the growing research in comprehending the metal microbe interactions in the presence of multimetal. This will facilitate in development of novel molecular markers for contaminant bioremediation.
dauerhafte UFZ-Verlinkung
Dey, P., Malik, A., Singh, D.K., Haange, S.-B., von Bergen, M., Jehmlich, N. (2022):
Insight to the molecular mechanisms underpinning the mycoremediation of multiple metals by proteomic technique
Front. Microbiol. 13 , art. 872576 10.3389/fmicb.2022.872576