Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1093/femsec/fiac034
Titel (primär) Enhanced perchloroethene dechlorination by humic acids via increasing the dehalogenase activity of Dehalococcoides strains
Autor Wan, J.; Chen, K.; Chen, J.; Qin, Z.; Adrian, L.; Shen, C.
Quelle FEMS Microbiology Ecology
Erscheinungsjahr 2022
Department UBT
Band/Volume 98
Heft 4
Seite von fiac034
Sprache englisch
Topic T7 Bioeconomy
Supplements https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/femsec/98/4/10.1093_femsec_fiac034/1/fiac034_supplemental_file.docx?Expires=1654610684&Signature=lk0dDKDDWLF3ZWk6GDgsBX3eb1CB8W5q0U-D7wtJxUBg5-YlgQWQPL3sGfhogTACZ0j9DaoW-COiNguLTwQKncxuGWY3ns4wB4U5Y0idTQpoiVpbn4y8xTq6lA-xt3qOWmkNmosfTkBO1M3z3ZRGlfB4iukIR9PITitm2x1FA09RA6RNs~TnCqDtRnnQAoSK6gyxxnPBzZJVjOWK9MckRMgEm0uNJ~bRFD58vnyUauoU8qCfWKJ9c4X1OjFVwuYirZUKzsnnRPFV9keWHZOEuYP9ZtprEJiMI6csT5YcDZf8UhH-dxT03DKTq4dzMnSfSEhhPLXLnKzj9fAUdD2Vxw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
Keywords bioremediation; dehalococcoides; enzymatic assays; humic acids; quinone-independent respiration; reductive dechlorination
Abstract Perchloroethene (PCE) is a widely used chlorinated solvent. PCE is toxic to humans and has been identified as an environmental contaminant at thousands of sites worldwide. Several Dehalococcoides mccartyi strains can transform PCE to ethene, and thus contribute to bioremediation of contaminated sites. Humic acids (HA) are ubiquitous redox-active compounds of natural aquatic and soil systems and have been intensively studied because of their effect in electron transfer. In this study, we observed the dechlorination of PCE was accelerated by HA in mixed cultures containing Dehalococcoides strains. Anthraquinone-2,6-disulfonic acid (AQDS), a humic acid analogue, inhibited PCE dechlorination in our cultures and thus induced an opposite effect on PCE dehalogenation than HA. We observed the same effect on PCE dechlorination with the pure culture of Dehalococcoides mccartyi strain CBDB1. Not only in mixed cultures but also in pure cultures, growth of Dehalococcoides was not influenced by HA but inhibited by AQDS. Enzymatic activity tests confirmed the dehalogenating activity of strain CBDB1 was increased by HA, especially when using hydrogen as electron donor. We conclude that HA enhanced PCE dechlorination by increasing the reaction speed between hydrogen and the dehalogenase enzyme rather than acting as electron shuttle through its quinone moieties.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=26086
Wan, J., Chen, K., Chen, J., Qin, Z., Adrian, L., Shen, C. (2022):
Enhanced perchloroethene dechlorination by humic acids via increasing the dehalogenase activity of Dehalococcoides strains
FEMS Microbiol. Ecol. 98 (4), fiac034 10.1093/femsec/fiac034