Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3389/fmicb.2022.1046260
Lizenz creative commons licence
Titel (primär) A unified and simple medium for growing model methanogens
Autor Dzofou Ngoumelah, D.; Harnisch, F. ORCID logo ; Sulheim, S.; Heggeset, T.M.B.; Aune, I.H.; Wentzel, A.; Kretzschmar, J.
Quelle Frontiers in Microbiology
Erscheinungsjahr 2023
Department UMB
Band/Volume 13
Seite von art. 1046260
Sprache englisch
Topic T7 Bioeconomy
Keywords methanogenic archaea; medium adaptation; microbial electrochemical technology; bioelectrochemical systems; doubling time; specific growth rate
Abstract Apart from their archetypic use in anaerobic digestion (AD) methanogenic archaea are targeted for a wide range of applications. Using different methanogenic archaea for one specific application requires the optimization of culture media to enable the growth of different strains under identical environmental conditions, e.g., in microbial electrochemical technologies (MET) for (bio)electromethanation. Here we present a new culture medium (BFS01) adapted from the DSM-120 medium by omitting resazurin, yeast extract, casitone, and using a low salt concentration, that was optimized for Methanosarcina barkeri, Methanobacterium formicicum, and Methanothrix soehngenii. The aim was to provide a medium for follow-up co-culture studies using specific methanogens and Geobacter spp. dominated biofilm anodes. All three methanogens showed growth and activity in the BFS01 medium. This was demonstrated by estimating the specific growth rates ( μ ) and doubling times ( td ) of each methanogen. The μ and td based on methane accumulation in the headspace showed values consistent with literature values for M. barkeri and M. soehngenii. However, μ and td based on methane accumulation in the headspace differed from literature data for M. formicicum but still allowed sufficient growth. The lowered salt concentration and the omission of chemically complex organic components in the medium may have led to the observed deviation from μ and td for M. formicicum as well as the changed morphology. 16S rRNA gene-based amplicon sequencing and whole genome nanopore sequencing further confirmed purity and species identity.
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Dzofou Ngoumelah, D., Harnisch, F., Sulheim, S., Heggeset, T.M.B., Aune, I.H., Wentzel, A., Kretzschmar, J. (2023):
A unified and simple medium for growing model methanogens
Front. Microbiol. 13 , art. 1046260 10.3389/fmicb.2022.1046260