Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Creative Commons Lizenz creative commons licence
Titel (primär) Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS
Autor Qin, W.; Stärk, H.-J.; Müller, S.; Reemtsma, T.
Journal / Serie Frontiers in Microbiology
Erscheinungsjahr 2022
Department UMB; ANA
Band/Volume 13
Seite von art. 870931
Sprache englisch
Topic T9 Healthy Planet
Supplements https://ndownloader.figstatic.com/files/34890654
Keywords Elemental distribution; Transition metals, Phosphorus; Absorption; Toxicity; Adsorption
Abstract The effect of six heavy metals, Ag, Pb, Pd, Cu, Ni and Cr, on P uptake by yeast was investigated by single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry (SC-ICP-TOF-MS). It was found that the P content in cells with 1.55 g L-1 P feeding after P starvation was increased by ~ 70 % compared to control cells. Heavy metals at 10 ppm, except Cu, had a negative impact on P accumulation by cells. Pd reduced the P content by 26 % in single cells compared to control cells. Metal uptake was strongest for Ag and Pd (0.7 × 10-12 L cell-1) and weakest for Cr (0.05 × 10-12 L cell-1). Exposure to Cr markedly reduced (- 50 %) Mg in cells and had the greatest impact on the intrinsic element composition. The SC-ICP-TOF-MS shows the diversity of elemental content in single cells: for example, the P content under standard conditions varied between 12.4 to 890 fg cell-1. This technique allows to study both, the uptake of elements and sublethal effects on the physiology at a single cell level.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25929
Qin, W., Stärk, H.-J., Müller, S., Reemtsma, T. (2022):
Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS
Front. Microbiol. 13 , art. 870931