Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.3389/fmicb.2022.870931 |
Lizenz  |
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| Titel (primär) | Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS |
| Autor | Qin, W.; Stärk, H.-J.; Müller, S.; Reemtsma, T. |
| Quelle | Frontiers in Microbiology |
| Erscheinungsjahr | 2022 |
| Department | UMB; ANA |
| Band/Volume | 13 |
| Seite von | art. 870931 |
| Sprache | englisch |
| Topic | T9 Healthy Planet T7 Bioeconomy |
| Supplements | https://ndownloader.figstatic.com/files/34890654 |
| Keywords | Elemental distribution; Transition metals, Phosphorus; Absorption; Toxicity; Adsorption |
| Abstract | The effect of six heavy metals, Ag, Pb, Pd, Cu, Ni and Cr, on P uptake by yeast was investigated by single-cell analysis using inductively coupled plasma time-of-flight mass spectrometry (SC-ICP-TOF-MS). It was found that the P content in cells with 1.55 g L-1 P feeding after P starvation was increased by ~ 70 % compared to control cells. Heavy metals at 10 ppm, except Cu, had a negative impact on P accumulation by cells. Pd reduced the P content by 26 % in single cells compared to control cells. Metal uptake was strongest for Ag and Pd (0.7 × 10-12 L cell-1) and weakest for Cr (0.05 × 10-12 L cell-1). Exposure to Cr markedly reduced (- 50 %) Mg in cells and had the greatest impact on the intrinsic element composition. The SC-ICP-TOF-MS shows the diversity of elemental content in single cells: for example, the P content under standard conditions varied between 12.4 to 890 fg cell-1. This technique allows to study both, the uptake of elements and sublethal effects on the physiology at a single cell level. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25929 |
| Qin, W., Stärk, H.-J., Müller, S., Reemtsma, T. (2022): Exploring the extent of phosphorus and heavy metal uptake by single cells of Saccharomyces cerevisiae and their effects on intrinsic elements by SC-ICP-TOF-MS Front. Microbiol. 13 , art. 870931 10.3389/fmicb.2022.870931 |
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