Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3389/fbioe.2022.855715
Lizenz creative commons licence
Titel (primär) Exploitation of hetero- and phototrophic metabolic modules for redox-intensive whole-cell biocatalysis
Autor Theodosiou, E.; Tüllinghoff, A.; Toepel, J.; Bühler, B.
Quelle Frontiers in Bioengineering and Biotechnology
Erscheinungsjahr 2022
Department SOMA
Band/Volume 10
Seite von art. 855715
Sprache englisch
Topic T7 Bioeconomy
Keywords whole-cell redox biocatalysis; central metabolism; TCA cycle; Metabolic Engineering; Cyanobacteria; redox balance
Abstract The successful realization of a sustainable manufacturing bioprocess and the maximization of its production potential and capacity are the main concerns of a bioprocess engineer. A main step towards this endeavor is the development of an efficient biocatalyst. Isolated enzyme(s), microbial cells, or (immobilized) formulations thereof can serve as biocatalysts. Living cells feature, beside active enzymes, metabolic modules that can be exploited to support energy-dependent and multi-step enzyme-catalyzed reactions. Metabolism can sustainably supply necessary cofactors or cosubstrates at the expense of readily available and cheap resources, rendering external addition of costly cosubstrates unnecessary. However, for the development of an efficient whole-cell biocatalyst, in depth comprehension of metabolic modules and their interconnection with cell growth, maintenance, and product formation is indispensable. In order to maximize the flux through biosynthetic reactions and pathways to an industrially relevant product and respective key performance indices (i.e., titer, yield, and productivity), existing metabolic modules can be redesigned and/or novel artificial ones established. This review focuses on whole-cell bioconversions that are coupled to heterotrophic or phototrophic metabolism and discusses metabolic engineering efforts aiming at 1) increasing regeneration and supply of redox equivalents, such as NAD(P/H), 2) blocking competing fluxes, and 3) increasing the availability of metabolites serving as (co)substrates of desired biosynthetic routes.
dauerhafte UFZ-Verlinkung
Theodosiou, E., Tüllinghoff, A., Toepel, J., Bühler, B. (2022):
Exploitation of hetero- and phototrophic metabolic modules for redox-intensive whole-cell biocatalysis
Front. Bioeng. Biotechnol. 10 , art. 855715 10.3389/fbioe.2022.855715