Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/v14020195
Lizenz creative commons licence
Titel (primär) Mycelia-assisted isolation of non-host bacteria able to co-transport phages
Autor You, X.; Klose, N.; Kallies, R.; Harms, H.; Chatzinotas, A.; Wick, L.Y.
Quelle Viruses
Erscheinungsjahr 2022
Department UMB; iDiv
Band/Volume 14
Heft 2
Seite von art. 195
Sprache englisch
Topic T7 Bioeconomy
Keywords viruses; co-transport; viral adsorption; motile bacteria; hitchhiking
Abstract Recent studies have demonstrated that phages can be co-transported with motile non-host bacteria, thereby enabling their invasion of biofilms and control of biofilm composition. Here, we developed a novel approach to isolate non-host bacteria able to co-transport phages from soil. It is based on the capability of phage-carrying non-host bacteria to move along mycelia out of soil and form colonies in plaques of their co-transported phages. The approach was tested using two model phages of differing surface hydrophobicity, i.e., hydrophobic Escherichia virus T4 (T4) and hydrophilic Pseudoalteromonas phage HS2 (HS2). The phages were mixed into soil and allowed to be transported by soil bacteria along the mycelia of Pythium ultimum. Five phage-carrying bacterial species were isolated (Viridibacillus sp., Enterobacter sp., Serratia sp., Bacillus sp., Janthinobacterium sp.). These bacteria exhibited phage adsorption efficiencies of ≈90–95% for hydrophobic T4 and 30–95% for hydrophilic HS2. The phage adsorption efficiency of Viridibacillus sp. was ≈95% for both phages and twofold higher than T4-or HS2-adsorption to their respective hosts, qualifying Viridibacillus sp. as a potential super carrier for phages. Our approach offers an effective and target-specific way to identify and isolate phage-carrying bacteria in natural and man-made environments.
dauerhafte UFZ-Verlinkung
You, X., Klose, N., Kallies, R., Harms, H., Chatzinotas, A., Wick, L.Y. (2022):
Mycelia-assisted isolation of non-host bacteria able to co-transport phages
Viruses 14 (2), art. 195 10.3390/v14020195