Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/plants10102092
Lizenz creative commons licence
Titel (primär) Molecular screening of microorganisms associated with discolored wood in dead European beech trees suffered from extreme drought event using next generation sequencing
Autor Purahong, W.; Tanunchai, B.; Wahdan, S.F.M.; Buscot, F.; Schulze, E.-D.
Quelle Plants
Erscheinungsjahr 2021
Department BOOEK; iDiv
Band/Volume 10
Heft 10
Seite von art. 2092
Sprache englisch
Topic T5 Future Landscapes
Supplements https://www.mdpi.com/2223-7747/10/10/2092/s1
Keywords climate change; Fagus sylvatica; Illumina sequencing; meta-barcoding; plant pathogenic bacteria; plant pathogenic fungi
Abstract Drought events weaken trees and make them vulnerable to attacks by diverse plant pathogens. Here, we propose a molecular method for fast screening of microorganisms associated with European beech decline after an extreme drought period (2018) in a forest of Thuringia, Germany. We used Illumina sequencing with a recent bioinformatics approach based on DADA2 to identify archaeal, bacterial, and fungal ASVs (amplicon sequence variants) based on bacterial and archaeal 16S and fungal ITS genes. We show that symptomatic beech trees are associated with both bacterial and fungal plant pathogens. Although the plant pathogen sequences were detected in both discolored and non-discolored wood areas, they were highly enriched in the discolored wood areas. We show that almost each individual tree was associated with a different combination of pathogens. Cytospora spp. and Neonectria coccinea were among the most frequently detected fungal pathogens, whereas Erwinia spp. and Pseudomonas spp. were the dominant bacterial plant pathogens. We demonstrate that bacterial plant pathogens may be of major importance in beech decline.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25140
Purahong, W., Tanunchai, B., Wahdan, S.F.M., Buscot, F., Schulze, E.-D. (2021):
Molecular screening of microorganisms associated with discolored wood in dead European beech trees suffered from extreme drought event using next generation sequencing
Plants 10 (10), art. 2092