Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Creative Commons Lizenz creative commons licence
Titel (primär) Different ways to play it cool: Transcriptomic analysis sheds light on different activity patterns of three amphipod species under long-term cold exposure
Autor Lipaeva, P.; Vereshchagina, K.; Drozdova, P.; Jakob, L.; Kondrateva, E.; Lucassen, M.; Bedulina, D.; Timofeyev, M.; Stadler, P.; Luckenbach, T.
Journal / Serie Molecular Ecology
Erscheinungsjahr 2021
Department BIOTOX
Band/Volume 30
Heft 22
Seite von 5735
Seite bis 5751
Sprache englisch
Topic T9 Healthy Planet
Supplements https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1111%2Fmec.16164&file=mec16164-sup-0001-SupInfo.doc
Keywords cold adaptation; transcriptome sequencing; Lake Baikal; freshwater lake; Gammarus lacustris; Eulimnogammarus verrucosus; Eulimnogammarus cyaneus
Abstract Species of littoral freshwater environments in regions with continental climate experience pronounced seasonal temperature changes. Coping with long cold winters and hot summers requires specific physiological and behavioral adaptations. Endemic amphipods of Lake Baikal, Eulimnogammarus verrucosus and Eulimnogammarus cyaneus, show high metabolic activity throughout the year; Everrucosus even reproduces in winter. In contrast, the widespread Holarctic amphipod Gammarus lacustris overwinters in torpor. This study investigated the transcriptomic hallmarks of Everrucosus, Ecyaneus and Glacustris exposed to low water temperatures. Amphipods were exposed to 1.5 °C and 12 °C (corresponding to the mean winter and summer water temperatures, respectively, in the Baikal littoral) for one month. At 1.5 °C, Glacustris showed up-regulation of ribosome biogenesis and mRNA processing genes, as well as down-regulation of genes related to growth, reproduction and locomotor activity, indicating enhanced energy allocation to somatic maintenance. Our results suggest that the mitogen‑activated protein kinase (MAPK) signaling pathway is involved in the preparation for hibernation; down-regulation of the actin cytoskeleton pathway genes could relate to the observed low locomotor activity of Glacustris at 1.5 °C. The differences between the transcriptomes of E. verrucosus and E. cyaneus from the 1.5 °C and 12 °C exposures were considerably smaller than for G. lacustris. In Everrucosus, cold-exposure triggered reproductive activity was indicated by up-regulation of respective genes, whereas in Ecyaneus, genes related to mitochondria functioning were up-regulated, indicating cold compensation in this species. Our data elucidate the molecular characteristics behind the different adaptations of amphipod species from the Lake Baikal area to winter conditions.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=25089
Lipaeva, P., Vereshchagina, K., Drozdova, P., Jakob, L., Kondrateva, E., Lucassen, M., Bedulina, D., Timofeyev, M., Stadler, P., Luckenbach, T. (2021):
Different ways to play it cool: Transcriptomic analysis sheds light on different activity patterns of three amphipod species under long-term cold exposure
Mol. Ecol. 30 (22), 5735 - 5751