Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Pseudomonas putida KT2440 responds specifically to chlorophenoxy herbicides and their initial metabolites
Autor Benndorf, D.; Thiersch, M.; Loffhagen, N.; Kunath, C.; Harms, H.;
Journal / Serie Proteomics
Erscheinungsjahr 2006
Department UMB; PROTEOM;
Band/Volume 6
Heft 11
Sprache englisch;
Keywords Chemostress; 2-D database; Multiple drug resistance; Oxidative stress; Resistance mechanism
Abstract Pseudomonas putida KT2440 is often used as a model to investigate toxicity mechanisms and adaptation to hazardous chemicals in bacteria. The objective of this paper was to test the impact of the chlorophenoxy herbicides 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-(2,4-dichlorophenoxy)propanoic acid (DCPP) and their metabolites 2,4-dichlorophenol (DCP) and 3,5-dichlorocatechol (DCC), on protein expression patterns and physiological parameters. Both approaches showed that DCC has a different mode of action and induces different responses than DCPP, 2,4-D and DCP. DCC was the most toxic compound and was active as an uncoupler of oxidative phosphorylation. It repressed the synthesis of ferric uptake regulator (Fur)-dependent proteins, e.g. fumarase C and L-ornithine N5-oxygenase, which are involved in oxidative stress response and iron uptake. DCPP, 2,4-D and DCP were less toxic than DCC. They disturbed oxidative phosphorylation to a lesser extent by a yet unknown mechanism. Furthermore, they repressed enzymes of energy-consuming biosynthetic pathways and induced membrane transporters for organic substrates. A TolC homologue component of multidrug resistance transporters was found to be induced, which is probably involved in the removal of lipophilic compounds from membranes.
ID 2496
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2496
Benndorf, D., Thiersch, M., Loffhagen, N., Kunath, C., Harms, H. (2006):
Pseudomonas putida KT2440 responds specifically to chlorophenoxy herbicides and their initial metabolites
Proteomics 6 (11), 3319 - 3329