Details zur Publikation

Referenztyp Zeitschriften
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Creative Commons Lizenz creative commons licence
Titel (primär) YB-1 is altered in pregnancy-associated disorders and affects trophoblast in vitro properties via alternation of multiple molecular traits
Autor Stojanovska, V.; Shah, A.; Woidacki, K.; Fischer, F.; Bauer, M.; Lindquist, J.A.; Mertens, P.R.; Zenclussen, A.C.;
Journal / Serie International Journal of Molecular Sciences
Erscheinungsjahr 2021
Department IMMU;
Band/Volume 22
Heft 13
Sprache englisch;
Supplements https://www.mdpi.com/1422-006 7/22/13/7226/s1
Keywords cold shock protein; intrauterine growth restriction; preeclampsia; placentation; apoptosis; NF-κB
Abstract Cold shock Y-box binding protein-1 (YB-1) coordinates several molecular processes between the nucleus and the cytoplasm and plays a crucial role in cell function. Moreover, it is involved in cancer progression, invasion, and metastasis. As trophoblast cells share similar characteristics with cancer cells, we hypothesized that YB-1 might also be necessary for trophoblast functionality. In samples of patients with intrauterine growth restriction, YB-1 mRNA levels were decreased, while they were increased in preeclampsia and unchanged in spontaneous abortions when compared to normal pregnant controls. Studies with overexpression and downregulation of YB-1 were performed to assess the key trophoblast processes in two trophoblast cell lines HTR8/SVneo and JEG3. Overexpression of YB-1 or exposure of trophoblast cells to recombinant YB-1 caused enhanced proliferation, while knockdown of YB-1 lead to proliferative disadvantage in JEG3 or HTR8/SVneo cells. The invasion and migration properties were affected at different degrees among the trophoblast cell lines. Trophoblast expression of genes mediating migration, invasion, apoptosis, and inflammation was altered upon YB-1 downregulation. Moreover, IL-6 secretion was excessively increased in HTR8/SVneo. Ultimately, YB-1 directly binds to NF-κB enhancer mark in HTR8/SVneo cells. Our data show that YB-1 protein is important for trophoblast cell functioning and, when downregulated, leads to trophoblast disadvantage that at least in part is mediated by NF-κB.
ID 24801
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24801
Stojanovska, V., Shah, A., Woidacki, K., Fischer, F., Bauer, M., Lindquist, J.A., Mertens, P.R., Zenclussen, A.C. (2021):
YB-1 is altered in pregnancy-associated disorders and affects trophoblast in vitro properties via alternation of multiple molecular traits
Int. J. Mol. Sci. 22 (13), art. 7226