Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s12072-022-10473-x
Lizenz creative commons licence
Titel (primär) Biomarkers of hepatocellular synthesis in patients with decompensated cirrhosis
Autor Gurbuz, B.; Guldiken, N.; Reuken, P.; Fu, L.; Remih, K.; Preisinger, C.; Brůha, R.; Leníček, M.; Petrtýl, J.; Reissing, J.; Aly, M.; Fromme, M.; Zhou, B.; Karkossa, I.; Schubert, K.; von Bergen, M.; Stallmach, A.; Bruns, T.; Strnad, P.
Journal / Serie Hepatology International
Erscheinungsjahr 2023
Department iDiv; MOLSYB
Band/Volume 17
Heft 3
Seite von 698
Seite bis 708
Sprache englisch
Topic T9 Healthy Planet
Keywords Mass spectrometry; Fibrosis; Interleukin; Hepatocyte nuclear factor; Decompensated cirrhosis

Background and aim

Since hepatocytes produce majority of serum proteins, patients with cirrhosis display substantial alterations in the serum proteome. The aim of the current study was to characterize these changes and to study the prognostic utility of hepatocellular proteins available in routine clinical testing.


Sera from 29 healthy controls and 43 patients with cirrhosis were subjected to untargeted proteomic analysis. Unsupervised hierarchical clustering was performed with Perseus software and R. Ingenuity pathway analysis (IPA) suggested upstream regulators that were validated in liver tissues. The behavior and prognostic usefulness of selected biomarkers was investigated in 61 controls and 285 subjects with decompensated cirrhosis.


Proteomics uncovered 65 and 16 hepatocellular serum proteins that are significantly downregulated or upregulated in patients with cirrhosis vs. controls. Hierarchical clustering revealed two main clusters and six sub-clusters. IPA identified HNF4α and IL-6 as the two major upstream regulators that were confirmed by hepatic gene expression analyses. Among pseudocholinesterase, transferrin, transthyretin, albumin, and apolipoprotein AI (Apo-AI), Apo-AI was the best predictor of 90-days transplant-free survival (AUROC 0.678; p = 0.0001) and remained an independent predictor in multivariable Cox independently of the presence of acute-on-chronic liver failure.


Our study reveals cirrhosis-associated changes in hepatocellular serum proteins and underlying transcription factors. Serum apolipoprotein AI may constitute a useful prognostic adjunct in patients with decompensated cirrhosis.

dauerhafte UFZ-Verlinkung
Gurbuz, B., Guldiken, N., Reuken, P., Fu, L., Remih, K., Preisinger, C., Brůha, R., Leníček, M., Petrtýl, J., Reissing, J., Aly, M., Fromme, M., Zhou, B., Karkossa, I., Schubert, K., von Bergen, M., Stallmach, A., Bruns, T., Strnad, P. (2023):
Biomarkers of hepatocellular synthesis in patients with decompensated cirrhosis
Hepatol. Int. 17 (3), 698 - 708