Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.21873/anticanres.14951 |
| Titel (primär) | Micronucleus formation in primary oropharyngeal epithelial cells reveals mutagenicity of cement dusts |
| Autor | Pawliczak, J.; Kolb, M.; Bauer, M.; Gminski, R.; Dietz, A.; Wichmann, G. |
| Quelle | Anticancer Research |
| Erscheinungsjahr | 2021 |
| Department | IMMU |
| Band/Volume | 41 |
| Heft | 4 |
| Seite von | 1849 |
| Seite bis | 1858 |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Keywords | Micronucleus assay; cement dust; oropharyngeal mucosa-derived primary epithelial cells |
| Abstract | Background/Aim: Epidemiological studies showed an increased risk of developing laryngeal head and neck squamous cell carcinoma (HNSCC) for employees working in the construction business. This suggested a causal link between exposure to cement particles and development of HNSCC but data were missing. Materials and Methods: We established an Organisation for Economic Co-operation and Development (OECD) guideline 487-conform micronucleus assay (MNA) using oropharyngeal mucosa-derived primary epithelial cells (OPCs) ex vivo. OPCs from healthy mucosa of 52 donors were cultured in vitro and incubated with serial concentrations of two common cement particles. Mitomycin C was used as a soluble positive control, and TiO2 and DQ12 were used as negative and positive particle controls. Bi-nucleated cells were counted and the mitotic index (MI) was determined. Subsequently, micronuclei-containing bi-nucleated cells (MN+) were counted. Results: Cement particles, in concentrations not significantly reducing ex vivo proliferation according to mitotic index, dose-dependently increased micronuclei formation. Conclusion: Through the establishment of an OECD guideline 487 conform MNA, we demonstrate the mutagenic effects of cement on human OPCs |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24575 |
| Pawliczak, J., Kolb, M., Bauer, M., Gminski, R., Dietz, A., Wichmann, G. (2021): Micronucleus formation in primary oropharyngeal epithelial cells reveals mutagenicity of cement dusts Anticancer Res. 41 (4), 1849 - 1858 10.21873/anticanres.14951 |
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