Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Micronucleus formation in primary oropharyngeal epithelial cells reveals mutagenicity of cement dusts
Autor Pawliczak, J.; Kolb, M.; Bauer, M.; Gminski, R.; Dietz, A.; Wichmann, G.;
Journal / Serie Anticancer Research
Erscheinungsjahr 2021
Department IMMU;
Band/Volume 41
Heft 4
Sprache englisch;
Keywords Micronucleus assay; cement dust; oropharyngeal mucosa-derived primary epithelial cells
Abstract Background/Aim: Epidemiological studies showed an increased risk of developing laryngeal head and neck squamous cell carcinoma (HNSCC) for employees working in the construction business. This suggested a causal link between exposure to cement particles and development of HNSCC but data were missing. Materials and Methods: We established an Organisation for Economic Co-operation and Development (OECD) guideline 487-conform micronucleus assay (MNA) using oropharyngeal mucosa-derived primary epithelial cells (OPCs) ex vivo. OPCs from healthy mucosa of 52 donors were cultured in vitro and incubated with serial concentrations of two common cement particles. Mitomycin C was used as a soluble positive control, and TiO2 and DQ12 were used as negative and positive particle controls. Bi-nucleated cells were counted and the mitotic index (MI) was determined. Subsequently, micronuclei-containing bi-nucleated cells (MN+) were counted. Results: Cement particles, in concentrations not significantly reducing ex vivo proliferation according to mitotic index, dose-dependently increased micronuclei formation. Conclusion: Through the establishment of an OECD guideline 487 conform MNA, we demonstrate the mutagenic effects of cement on human OPCs
ID 24575
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24575
Pawliczak, J., Kolb, M., Bauer, M., Gminski, R., Dietz, A., Wichmann, G. (2021):
Micronucleus formation in primary oropharyngeal epithelial cells reveals mutagenicity of cement dusts
Anticancer Res. 41 (4), 1849 - 1858