Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.21307/jofnem-2021-034
Lizenz creative commons licence
Titel (primär) Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece
Autor Tzortzakakis, E.A.; Clavero-Camacho, I.; Cantalapiedra-Navarrete, C.; Ralli, P.; Palomares-Rius, J.E.; Castillo, P.; Archidona-Yuste, A.
Quelle Journal of Nematology
Erscheinungsjahr 2021
Department OESA
Band/Volume 53
Seite von e2021-34
Sprache englisch
Topic T5 Future Landscapes
Keywords Cytochrome oxidase c subunit 1; D2-D3 of 28S rRNA; Description; ITS1 rRNA; Longidorus; L. pauli; L. pisi; Needle nematodes; Taxonomy
Abstract Sampling for needle nematodes was carried out in a grapevine area in Thessaloniki, North Greece and two nematode species of Longidorus (L. pauli and L. pisi) were collected. Nematodes were extracted from 500 cm3 of soil by modified sieving and decanting method, processed to glycerol and mounted on permanent slides, and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted to amplify D2-D3 expansion segments of 28S rRNA, ITS1 rRNA, and partial mitochondrial coxI regions. Morphology and morphometry data obtained from these populations were consistent with L. pauli and L. pisi identifications. To our knowledge, this is the first report of L. pauli for Greece, and the second world report after the original description from Idleb, Syria, extending the geographical distribution of this species in the Mediterranean Basin.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24516
Tzortzakakis, E.A., Clavero-Camacho, I., Cantalapiedra-Navarrete, C., Ralli, P., Palomares-Rius, J.E., Castillo, P., Archidona-Yuste, A. (2021):
Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece
J. Nematol. 53 , e2021-34 10.21307/jofnem-2021-034