Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1038/s41598-021-85594-8
Lizenz creative commons licence
Titel (primär) New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
Autor Greinert, T.; Vogel, K.; Maskow, T. ORCID logo ; Held, C.
Quelle Scientific Reports
Erscheinungsjahr 2021
Department UMB
Band/Volume 11
Seite von art. 6125
Sprache englisch
Topic T7 Bioeconomy
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41598-021-85594-8/MediaObjects/41598_2021_85594_MOESM1_ESM.pdf
Abstract Thermodynamic feasibility analyses help evaluating the feasibility of metabolic pathways. This is an important information used to develop new biotechnological processes and to understand metabolic processes in cells. However, literature standard data are uncertain for most biochemical reactions yielding wrong statements concerning their feasibility. In this article we present activity-based equilibrium constants for all the ten glycolytic reactions, accompanied by the standard reaction data (standard Gibbs energy of reaction and standard enthalpy of reaction). We further developed a thermodynamic activity-based approach that allows to correctly determine the feasibility of glycolysis under different chosen conditions. The results show for the first time that the feasibility of glycolysis can be explained by thermodynamics only if (1) correct standard data are used and if (2) the conditions in the cell at non-equilibrium states are accounted for in the analyses. The results here will help to determine the feasibility of other metabolisms and to understand metabolic processes in cells in the future.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24417
Greinert, T., Vogel, K., Maskow, T., Held, C. (2021):
New thermodynamic activity-based approach allows predicting the feasibility of glycolysis
Sci. Rep. 11 , art. 6125 10.1038/s41598-021-85594-8