Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1002/cyto.a.20212 |
| Titel (primär) | Analysis of living S. cerevisiae cell states - a three color approach |
| Autor | Achilles, J.; Harms, H.; Müller, S. |
| Journal / Serie | Cytometry Part A |
| Erscheinungsjahr | 2006 |
| Department | UMB |
| Band/Volume | 69A |
| Heft | 3 |
| Seite von | 173 |
| Seite bis | 177 |
| Sprache | englisch |
| Keywords | multiparametric flow cytometry; Hoechst 33342; 2-NBD-glucose; viability; population dynamics; metabolic modes; Saccharomyces cerevisiae |
| Abstract | Background: Biosyntheses often fluctuate with the state of the cell in the cell cycle and on the capacity of the cell to access and metabolize a carbon source, Visualization of Substrate uptake by individual cells, together with the simultaneous analysis of proliferation activity and the proportion of dead cells, facilitate reliable and quasi-online process optimization. Methods: Flow cytometry and Hoechst 33342 staining were used to follow proliferation activity of living Saccha romyces cerevisiae cells, whereas 2-NBD-glucose was employed to analyze the cells' substrate affinity. Propidium iodide was used to determine the proportion of dead cells. Calibration and verification experiments were performed with cells grown batch-wise as well as in transient state regimes. Results: A new and rapid three-color assay was developed and tested under varying microenvironmental conditions. Conclusions: Live/dead cell states and the affinity to 2-NBD-glucose vs. proliferation states were determined during respiratory and/or fermentative modes of metabolism. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2441 |
| Achilles, J., Harms, H., Müller, S. (2006): Analysis of living S. cerevisiae cell states - a three color approach Cytom. Part A 69A (3), 173 - 177 |
|