Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00216-020-03131-4
Lizenz creative commons licence
Titel (primär) Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo
Autor Halbach, K.; Holbrook, T.; Reemtsma, T.; Wagner, S.
Quelle Analytical and Bioanalytical Chemistry
Erscheinungsjahr 2021
Department ANA
Band/Volume 413
Heft 6
Seite von 1675
Seite bis 1687
Sprache englisch
Topic T9 Healthy Planet
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-020-03131-4/MediaObjects/216_2020_3131_MOESM1_ESM.pdf
https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-020-03131-4/MediaObjects/216_2020_3131_MOESM2_ESM.pdf
Keywords MSimaging; Toxicokinetics; Biological samples; Cluster analysis; ICP-ToF-MS; Organ
Abstract A workflow was developed and implemented in a software tool for the automated combination of spatially resolved laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) data and data on the morphology of the biological tissue. Making use of a recently published biological annotation software, FishImager automatically assigns the biological feature as regions of interest (ROIs) and overlays them with the quantitative LA-ICP-MS data. Furthermore, statistical tools including cluster algorithms can be applied to the elemental intensity data and directly compared with the ROIs. This is effectively visualized in heatmaps. This allows gaining statistical significance on distribution and co-localization patterns. Finally, the biological functions of the assigned ROIs can then be easily linked with elemental distributions. We demonstrate the versatility of FishImager with quantitative LA-ICP-MS data of the zebrafish embryo tissue. The distribution of natural elements and xenobiotics is analyzed and discussed. With the help of FishImager, it was possible to identify compartments affected by toxicity effects or biological mechanisms to eliminate the xenobiotic. The presented workflow can be used for clinical and ecotoxicological testing, for example. Ultimately, it is a tool to simplify and reproduce interpretations of imaging LA-ICP-MS data in many applications.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24125
Halbach, K., Holbrook, T., Reemtsma, T., Wagner, S. (2021):
Effective processing and evaluation of chemical imaging data with respect to morphological features of the zebrafish embryo
Anal. Bioanal. Chem. 413 (6), 1675 - 1687 10.1007/s00216-020-03131-4