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Titel (primär) Screening and cultivating microbial strains able to grow on building blocks of polyurethane
Titel (sekundär) Enzymatic plastic degradation
Autor Cárdenas Espinosa, M.J.; Colina Blanco, A.; Heipieper, H.J.; Eberlein, C.;
Herausgeber Weber, G.; Bornscheuer, U.T.; Wei, R.;
Journal / Serie Methods in Enzymology
Erscheinungsjahr 2021
Department UBT;
Band/Volume 648
Sprache englisch;
POF III (gesamt) T15;
Keywords Polyurethane; PU building blocks; Biodegradation; TDA; MDA; Isolation of bacteria
Abstract The diverse benefits of synthetic polymers is overshadowed by the amount of plastic waste and its whereabouts. The problem can only be tackled by reducing and recycling of plastics. In this respect, investigating the (microbial) degradation of each type of polymer currently used may provide further understanding that fosters the development of new feasible recycling technologies. Here, we present a strategy to isolate bacteria from environmental samples that are able to degrade hydrolysis products and building blocks of polyurethane (PUR). Protocols are presented to enrich bacteria on the primary diamines 2,4-diaminotoluene (TDA) and 4,4′-diaminodiphenylmethane (MDA) as well as an oligomeric PUR (Sigma Aldrich, proprietary composition). For TDA and the oligomeric PUR, methods are suggested to monitor their concentration in bacterial enrichment cultures.
ID 24084
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=24084
Cárdenas Espinosa, M.J., Colina Blanco, A., Heipieper, H.J., Eberlein, C. (2021):
Screening and cultivating microbial strains able to grow on building blocks of polyurethane
In: Weber, G., Bornscheuer, U.T., Wei, R. (eds.)
Enzymatic plastic degradation
Methods Enzymol. 648
Elsevier, p. 423 - 434