Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Thermodynamics and kinetics of glycolytic reactions. Part II: Influence of cytosolic conditions on thermodynamic state variables and kinetic parameters
Autor Vogel, K.; Greinert, T.; Reichard, M.; Held, C.; Harms, H.; Maskow, T.;
Journal / Serie International Journal of Molecular Sciences
Erscheinungsjahr 2020
Department UMB;
Band/Volume 21
Heft 21
Sprache englisch;
POF III (gesamt) T41;
Supplements https://www.mdpi.com/1422-0067/21/21/7921/s1
Keywords biothermodynamics; enzyme kinetics; glycolysis; isothermal titration calorimetry; macromolecular crowding
Abstract For systems biology, it is important to describe the kinetic and thermodynamic properties of enzyme-catalyzed reactions and reaction cascades quantitatively under conditions prevailing in the cytoplasm. While in part I kinetic models based on irreversible thermodynamics were tested, here in part II, the influence of the presumably most important cytosolic factors was investigated using two glycolytic reactions (i.e., the phosphoglucose isomerase reaction (PGI) with a uni-uni-mechanism and the enolase reaction with an uni-bi-mechanism) as examples. Crowding by macromolecules was simulated using polyethylene glycol (PEG) and bovine serum albumin (BSA). The reactions were monitored calorimetrically and the equilibrium concentrations were evaluated using the equation of state ePC-SAFT. The pH and the crowding agents had the greatest influence on the reaction enthalpy change. Two kinetic models based on irreversible thermodynamics (i.e., single parameter flux-force and two-parameter Noor model) were applied to investigate the influence of cytosolic conditions. The flux-force model describes the influence of cytosolic conditions on reaction kinetics best. Concentrations of magnesium ions and crowding agents had the greatest influence, while temperature and pH-value had a medium influence on the kinetic parameters. With this contribution, we show that the interplay of thermodynamic modeling and calorimetric process monitoring allows a fast and reliable quantification of the influence of cytosolic conditions on kinetic and thermodynamic parameters.
ID 23833
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23833
Vogel, K., Greinert, T., Reichard, M., Held, C., Harms, H., Maskow, T. (2020):
Thermodynamics and kinetics of glycolytic reactions. Part II: Influence of cytosolic conditions on thermodynamic state variables and kinetic parameters
Int. J. Mol. Sci. 21 (21), art. 7921