Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Bioanalytical equivalents and relative potencies for predicting the biological effects of mixtures
Autor Zhou, S.; Peng, S.; Brack, W.; Doering, J.A.; Seiler, T.-B.; Hollert, H.;
Journal / Serie Science of the Total Environment
Erscheinungsjahr 2021
Department WANA;
Band/Volume 763
Sprache englisch;
POF III (gesamt) T32;
Keywords 2,3,7,8-TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; EXR: 7-ethoxyresorufin; AhR: aryl hydrocarbon receptor; BEQ: bioanalytical equivalent; Bio-BEQ: bioassay-detected BEQ; Chem-BEQ: chemically analyzed BEQ; CA model: concentration addition model; CALUX: Chemically Activated Luciferase gene eXpression; EROD: 7-ethoxyresorufin-o-deethylase; IA model: independent action model; REP: relative potency; NF-κB: Nuclear Factor Kappa B; MoA: modes of action; PAH: polycyclic aromatic hydrocarbon; TEF: toxicity equivalency factor
Abstract Bioanalytical equivalents (BEQs) of mixtures and environmental samples are widely used to reflect the potential threat of pollutants in the environment and can be obtained by bioassays or using chemical analysis combined with relative potencies (REPs). In this study, the relationships between bioassay-detected BEQs (Bio-BEQs) and chemically analyzed BEQs (Chem-BEQs) were studied. BEQs and REPs are correlated with effect level and the concentration-response curves of the reference standard and sample. Thus, effect level (e.g., EC10, EC25 and EC50) should be addressed for the BEQ values obtained from bioassays or chemical analyses. The previous prerequisites for REPs application (i.e., curves that are parallel and have the same maximum response) are redundant, and the use of REPs for the calculation of BEQs or in risk assessment should instead be based on the same effect level. For a complex mixture with many components, all active components can be regarded as dilutions of a standard compound for inducing a specific effect. Relative toxicity estimates based on EC50 ignore the contribution of weak-active components with maximum response below EC50 of the reference standard, especially in complex mixtures or environmental samples. REPs based on an effect level EC10 that can be clearly discriminated from background response are recommended for BEQ calculation. As an example, the aryl hydrocarbon receptor (AhR)-mediated activity of US EPA priority polycyclic aromatic hydrocarbons (PAHs) in RTL-W1 cells was used to assess the reliability of REPs for mixture toxicity prediction based on the effect level EC10.
ID 23800
dauerhafte UFZ-Verlinkung
Zhou, S., Peng, S., Brack, W., Doering, J.A., Seiler, T.-B., Hollert, H. (2021):
Bioanalytical equivalents and relative potencies for predicting the biological effects of mixtures
Sci. Total Environ. 763 , art. 143030