Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/microorganisms11040837
Lizenz creative commons licence
Titel (primär) Assessment of new and genome-reduced Pseudomonas strains regarding their robustness as chassis in biotechnological applications
Autor Cárdenas Espinosa, M.J.; Schmidgall, T.; Pohl, J.; Wagner, G.; Wynands, B.; Wierckx, N.; Heipieper, H.J. ORCID logo ; Eberlein, C. ORCID logo
Quelle Microorganisms
Erscheinungsjahr 2023
Department UBT
Band/Volume 11
Heft 4
Seite von art. 837
Sprache englisch
Topic T7 Bioeconomy
Keywords Pseudomonas; biocatalysts; genome-reduced chassis; bio-production
Abstract Organic olvent-tolerant strains of the Gram-negative bacterial genus Pseudomonas are discussed as potential biocatalysts for the biotechnological production of various chemicals. However, many current strains with the highest tolerance are belonging to the species P. putida and are classified as biosafety level 2 strains, which makes them uninteresting for the biotechnological industry. Therefore, it is necessary to identify other biosafety level 1 Pseudomonas strains with high tolerance towards solvents and other forms of stress, which are suitable for establishing production platforms of biotechnological processes. In order to exploit the native potential of Pseudomonas as a microbial cell factory, the biosafety level 1 strain P. taiwanensis VLB120 and its genome-reduced chassis (GRC) variants as well as the plastic-degrading strain P. capeferrum TDA1 were assessed regarding their tolerance towards different n-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol). Toxicity of the solvents was investigated by their effects on bacterial growth rates given as the EC50 concentrations. Hereby, both toxicities as well as the adaptive responses of P. taiwanensis GRC3 and P. capeferrum TDA1 showed EC50 values up to two-fold higher than those previously detected for P. putida DOT-T1E (biosafety level 2), one of the best described solvent-tolerant bacteria. Furthermore, in two-phase solvent systems, all the evaluated strains were adapted to 1-decanol as a second organic phase (i.e., OD560 was at least 0.5 after 24 h of incubation with 1% (v/v) 1-decanol), which shows the potential use of these strains as platforms for the bio-production of a wide variety of chemicals at industrial level.
dauerhafte UFZ-Verlinkung
Cárdenas Espinosa, M.J., Schmidgall, T., Pohl, J., Wagner, G., Wynands, B., Wierckx, N., Heipieper, H.J., Eberlein, C. (2023):
Assessment of new and genome-reduced Pseudomonas strains regarding their robustness as chassis in biotechnological applications
Microorganisms 11 (4), art. 837 10.3390/microorganisms11040837