Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/microorganisms8091436
Lizenz creative commons licence
Titel (primär) Environmentally relevant concentration of bisphenol S shows slight effects on SIHUMIx
Autor Schäpe, S.S.; Krause, J.L.; Masanetz, R.K.; Riesbeck, S.; Starke, R.; Rolle-Kampczyk, U.; Eberlein, C. ORCID logo ; Heipieper, H.J. ORCID logo ; Herberth, G. ORCID logo ; von Bergen, M.; Jehmlich, N. ORCID logo
Quelle Microorganisms
Erscheinungsjahr 2020
Department UBT; IMMU; MOLSYB
Band/Volume 8
Heft 9
Seite von art. 1436
Sprache englisch
Keywords in vitro model; bisphenol S; metaproteomics; short-chain fatty acids; fatty acid methyl ester; intestinal microbiota
Abstract Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of polycarbonate plastics and epoxy resins and thus can be found in various plastic products and thermal papers. The microbiota disrupting effect of BPS on the community structure of the microbiome has already been reported, but little is known on how BPS affects bacterial activity and function. To analyze these effects, we cultivated the simplified human intestinal microbiota (SIHUMIx) in bioreactors at a concentration of 45 µM BPS. By determining biomass, growth of SIHUMIx was followed but no differences during BPS exposure were observed. To validate if the membrane composition was affected, fatty acid methyl esters (FAMEs) profiles were compared. Changes in the individual membrane fatty acid composition could not been described; however, the saturation level of the membranes slightly increased during BPS exposure. By applying targeted metabolomics to quantify short-chain fatty acids (SCFA), it was shown that the activity of SIHUMIx was unaffected. Metaproteomics revealed temporal effect on the community structure and function, showing that BPS has minor effects on the structure or functionality of SIHUMIx.
dauerhafte UFZ-Verlinkung
Schäpe, S.S., Krause, J.L., Masanetz, R.K., Riesbeck, S., Starke, R., Rolle-Kampczyk, U., Eberlein, C., Heipieper, H.J., Herberth, G., von Bergen, M., Jehmlich, N. (2020):
Environmentally relevant concentration of bisphenol S shows slight effects on SIHUMIx
Microorganisms 8 (9), art. 1436 10.3390/microorganisms8091436