Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1128/mSphere.00016-20 |
Lizenz  |
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| Titel (primär) | Inhibition of respiration of Candida albicans by small molecules increases phagocytosis efficacy by macrophages |
| Autor | Cui, S.; Li, M.; Hassan, R.Y.A.; Heintz-Buschart, A.; Wang, J.; Bilitewski, U. |
| Quelle | mSphere |
| Erscheinungsjahr | 2020 |
| Department | BOOEK; iDiv |
| Band/Volume | 5 |
| Seite von | e00016-20 |
| Sprache | englisch |
| Supplements | https://msphere.asm.org/content/msph/5/2/e00016-20/DC1/embed/inline-supplementary-material-1.tif?download=true https://msphere.asm.org/content/msph/5/2/e00016-20/DC2/embed/inline-supplementary-material-2.tif?download=true https://msphere.asm.org/content/msph/5/2/e00016-20/DC3/embed/inline-supplementary-material-3.docx?download=true https://msphere.asm.org/content/msph/5/2/e00016-20/DC4/embed/inline-supplementary-material-4.tif?download=true https://msphere.asm.org/content/msph/5/2/e00016-20/DC5/embed/inline-supplementary-material-5.csv?download=true |
| Keywords | complex III; respiratory chain; antimycin A; phagocytosis; metabolism |
| Abstract | Candida albicans adapts to various conditions in different body niches by regulating gene expression, protein synthesis, and metabolic pathways. These adaptive reactions not only allow survival but also influence the interaction with host cells, which is governed by the composition and structure of the fungal cell wall. Numerous studies had shown linkages between mitochondrial functionality, cell wall integrity and structure, and pathogenicity. Thus, we decided to inhibit single complexes of the respiratory chain of C. albicans and to analyze the resultant interaction with macrophages via their phagocytic activity. Remarkably, inhibition of the fungal bc1 complex by antimycin A increased phagocytosis, which correlated with an increased accessibility of β-glucans. To contribute to mechanistic insights, we performed metabolic studies, which highlighted significant changes in the abundance of constituents of the plasma membrane. Collectively, our results reinforce the strong linkage between fungal energy metabolism and other components of fungal physiology, which also determine the vulnerability to immune defense reactions. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23469 |
| Cui, S., Li, M., Hassan, R.Y.A., Heintz-Buschart, A., Wang, J., Bilitewski, U. (2020): Inhibition of respiration of Candida albicans by small molecules increases phagocytosis efficacy by macrophages mSphere 5 , e00016-20 10.1128/mSphere.00016-20 |
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