Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Creative Commons Lizenz creative commons licence
Titel (primär) Direct sample introduction GC-MS/MS for quantification of organic chemicals in mammalian tissues and blood extracted with polymers without clean-up
Autor Baumer, A.; Escher, B.I.; Landmann, J.; Ulrich, N.;
Journal / Serie Analytical and Bioanalytical Chemistry
Erscheinungsjahr 2020
Department AUC; ZELLTOX;
Band/Volume 412
Sprache englisch;
POF III (gesamt) T42;
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00216-020-02864-6/MediaObjects/216_2020_2864_MOESM1_ESM.pdf
Keywords Mammalian tissues; Direct sample introduction; GC-MS/MS; Passive sampling; PDMS; Co-extracted matrix; Reduction of matrix effects
Abstract Solvent extracts of mammalian tissues and blood contain a large amount of co-extracted matrix components, in particular lipids, which can adversely affect instrumental analysis. Clean-up typically degrades non-persistent chemicals. Alternatively, passive sampling with the polymer polydimethylsiloxane (PDMS) has been used for a comprehensive extraction from tissue without altering the mixture composition. Despite a smaller fraction of matrix being co-extracted by PDMS than by solvent extraction, direct analysis of PDMS extracts was only possible with direct sample introduction (DSI) GC-MS/MS, which prevented co-extracted matrix components entering the system. Limits of quantitation (LOQ) ranged from 4 to 20 pg μL−1 ethyl acetate (PDMS extract) for pesticides and persistent organic pollutants (POPs). The group of organophosphorus flame retardants showed higher LOQs up to 107 pg μL−1 due to sorption to active sites at the injection system. Intraday precision ranged between 1 and 10%, while the range of interday precision was between 1 and 18% depending on the analyte. The method was developed using pork liver, brain, and fat as well as blood and was then applied to analyze human post-mortem tissues where polychlorinated biphenyls (PCBs) as well as dichlorodiphenyltrichloroethane (DDT) and DDT metabolites were detected.
ID 23465
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23465
Baumer, A., Escher, B.I., Landmann, J., Ulrich, N. (2020):
Direct sample introduction GC-MS/MS for quantification of organic chemicals in mammalian tissues and blood extracted with polymers without clean-up
Anal. Bioanal. Chem. 412 , 7295 - 7305