Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1021/acs.est.0c02068
Volltext Autorenversion
Titel (primär) Yolk sac of zebrafish embryos as backpack for chemicals?
Autor Halbach, K.; Ulrich, N.; Goss, K.-U.; Seiwert, B.; Wagner, S.; Scholz, S. ORCID logo ; Luckenbach, T. ORCID logo ; Bauer, C.; Schweiger, N.; Reemtsma, T.
Quelle Environmental Science & Technology
Erscheinungsjahr 2020
Department BIOTOX; AUC; ANA
Band/Volume 54
Heft 16
Seite von 10159
Seite bis 10169
Sprache englisch
Keywords Partition coefficient; Kinetic modeling; Equilibrium; Zebrafish; Kinetics
Abstract The zebrafish embryo (Danio rerio) has developed into one of the most important nonsentient animal models for the hazard assessments of chemicals, but the processes governing its toxicokinetics (TK) are poorly understood. This study compares the uptake of seven test compounds into the embryonic body and the yolk sac of the zebrafish embryo using TK experiments, a dialysis approach, thermodynamic calculations, and kinetic modeling. Experimental data show that between 95% (4-iodophenol) and 67% (carbamazepine) of the total internal amount in 26 h post fertilization (hpf) embryos and between 80 and 49% in 74 hpf embryos were found in the yolk. Thus, internal concentrations determined for the whole embryo overestimate the internal concentration in the embryonic body: for the compounds of this study, up to a factor of 5. Partition coefficients for the embryonic body and a one-compartment model with diffusive exchange were calculated for the neutral test compounds and agreed reasonably with the experimental data. For prevalently ionic test compounds at exposure pH (bromoxynil, paroxetine), however, the extent and the speed of uptake were low and could not be modeled adequately. A better understanding of the TK of ionizable test compounds is essential to allow assessment of the validity of this organismic test system for ionic test compounds.
dauerhafte UFZ-Verlinkung
Halbach, K., Ulrich, N., Goss, K.-U., Seiwert, B., Wagner, S., Scholz, S., Luckenbach, T., Bauer, C., Schweiger, N., Reemtsma, T. (2020):
Yolk sac of zebrafish embryos as backpack for chemicals?
Environ. Sci. Technol. 54 (16), 10159 - 10169 10.1021/acs.est.0c02068