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Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.bbagen.2020.129675
Volltext Akzeptiertes Manuskript
Titel (primär) Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van ‘t HOFF
Autor Vogel, K.; Greinert, T.; Harms, H.; Sadowski, G.; Held, C.; Maskow, T. ORCID logo
Quelle Biochimica et Biophysica Acta-General Subjects
Erscheinungsjahr 2020
Department UMB
Band/Volume 1864
Heft 10
Seite von art. 129675
Sprache englisch
Keywords Biothermodynamics; Glycolysis; Macromolecular crowding; Isothermal titration calorimetry (ITC); ePC-SAFT; Enolase
Abstract Background
Thermodynamic methods are finding more and more applications in systems biology, which attempts to understand cell functions mechanistically. Unfortunately, the state variables used (reaction enthalpy and Gibbs energy) do not take sufficient account of the conditions inside of cells, especially the crowding with macromolecules.
Methods
For this reason, the influence of crowding agents and various other parameters such as salt concentrations, pH and temperature on equilibrium position and reaction enthalpy of the glycolytic example reaction 9 (2-Phospoglycerate - > Phosphoenolpyruvate + H2O) was investigated. The conditions were chosen to be as close as possible to the cytosolic conditions. Poly(ethylene glycol) MW = 20,000 g mol−1 (PEG 20,000) was used to analyze the influence of crowding with macromolecules. The equation of state electrolyte Perturbed-Chain Statistical Associating Fluid Theory (ePC-SAFT) was applied to consider the influence of crowding agents on the reaction equilibria.
Results and conclusions
For the reaction enthalpies and for the equilibria, it was found that the influence of salts and temperature is not pronounced while that of pH and PEG 20,000 concentration is considerable. Furthermore, it could be shown that under identical measurement conditions there are no differences between the van ‘t Hoff and the calorimetrically determined reaction enthalpy.
General significance
The results show how important it is to consider the special cytosolic conditions when applying thermodynamic data in systems biology.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23282
Vogel, K., Greinert, T., Harms, H., Sadowski, G., Held, C., Maskow, T. (2020):
Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van ‘t HOFF
Biochim. Biophys. Acta-Gen. Subj. 1864 (10), art. 129675 10.1016/j.bbagen.2020.129675