Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Manganese-oxidizing bacteria form multiple cylindrospermopsin transformation products with reduced human liver cell toxicity
Autor Martínez-Ruiz, E.B.; Cooper, M.; Al-Zeer, M.A.; Kurreck, J.; Adrian, L.; Szewzyk, U.;
Journal / Serie Science of the Total Environment
Erscheinungsjahr 2020
Department ISOBIO; UBT;
Band/Volume 729
Sprache englisch;
POF III (gesamt) T41;
Keywords Cyanotoxins; HepG2; HepaRG; Biogenic oxides; Mass spectrometry; Degradation
Abstract Cylindrospermopsin (CYN) is a toxic alkaloid highly persistent in aquatic environments. Biological removal of CYN was described previously. However, no transformation products formed by biological processes could be identified so far. Here, we describe that various manganese-oxidizing bacteria (MOB) transform CYN completely at an initial mean concentration of 7 mg L−1 (17 μM) within 3 to 34 days. Regardless of the strain, and transformation rate, transformation of CYN by MOB led to the same seven transformation products identified by mass spectrometry, which suggests that the removal of CYN by MOB follows a similar mechanism. Oxidation was the main transformation process, and the uracil moiety was the most susceptible part of the CYN molecule. In vitro cytotoxicity tests with the transformation products of CYN formed by one of the tested strains against the two human liver cell lines HepG2 and HepaRG, revealed that the transformation products were substantially less toxic than pure CYN for both cell lines. The results suggest that incubation with MOB might be an option for water treatment to remove CYN and may allow more detailed studies on the fate of CYN in the environment.
ID 23171
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=23171
Martínez-Ruiz, E.B., Cooper, M., Al-Zeer, M.A., Kurreck, J., Adrian, L., Szewzyk, U. (2020):
Manganese-oxidizing bacteria form multiple cylindrospermopsin transformation products with reduced human liver cell toxicity
Sci. Total Environ. 729 , art. 138924