Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1002/cyto.a.20402
Titel (primär) Limits of propidium iodide as a cell viability indicator for environmental bacteria
Autor Shi, L.; Günther, S.; Hübschmann, T.; Wick, L.Y.; Harms, H.; Müller, S.
Quelle Cytometry Part A
Erscheinungsjahr 2007
Department UMB
Band/Volume 71A
Heft 8
Seite von 592
Seite bis 598
Sprache englisch
Keywords multiparametric flow cytometry; bacterial viability; population dynamics; live/dead staining; propidium iodide
Abstract Background: Viability measurements of individual bacteria are applied in various scopes of research and industry using approaches where propidium iodide (PI) serves as dead cell indicator. The reliability of PI uptake as a cell viability indicator for dead (PI permeable) and viable (PI impermeable) bacteria was tested using two soil bacteria, the gram-Sphingomonas sp. LB126 and the gram+Mycobacterium frederiksbergense LB501T. Methods: Bacterial proliferation activities observed viaDAPI and Hoechst 33342 staining were linked to the energy charge and the proportion of dead cells as obtained by diOC6 (3)-staining and PI-uptake, respectively. Calibration and verification experiments were performed using batch cultures grown on different substrates. Results: PI uptake depended on the physiological state of the bacterial cells. Unexpectedly, up to 40% of both strains were stained by PI during early exponential growth on glucose when compared to 2-5% of cells in the early stationary phase of growth. Conclusions: The results question the utility of PI as a universal indicator for the viability of (environmental) bacteria. It rather appears that in addition to nonviable cells, PI also stains growing cells of Sphingomonas sp. and M. frederiksbergense during a short period of their life cycle. © 2007 International Society for Analytical Cytology
dauerhafte UFZ-Verlinkung
Shi, L., Günther, S., Hübschmann, T., Wick, L.Y., Harms, H., Müller, S. (2007):
Limits of propidium iodide as a cell viability indicator for environmental bacteria
Cytom. Part A 71A (8), 592 - 598 10.1002/cyto.a.20402