Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Volltext Akzeptiertes Manuskript
Titel (primär) Enrichment and identification of small proteins in a simplified human gut microbiome
Autor Petruschke, H.; Anders, J.; Stadler, P.F.; Jehmlich, N.; von Bergen, M.;
Journal / Serie Journal of Proteomics
Erscheinungsjahr 2020
Department MOLSYB;
Band/Volume 213
Sprache englisch;
POF III (gesamt) T42;
Keywords Small proteins (sProteins); Human gut microbiome; Proteomics
Abstract Small proteins (sProteins) with a size of 100 amino acids and less are involved in major biological processes and play an important role in different bacteria. Despite the increasing interest in them, the data on sProteins in bacterial communities, like the human gut microbiome is sparse. In this study, we are using the extended simplified human intestinal microbiota (SIHUMIx) as model system of the human gut microbiome to detect sProteins and to compare different sProtein enrichment methods. We observed that with our tested methods, the C8-cartridge enrichment resulted in the highest number of detected sProteins (n = 295) with high reproducibility among replication analysis. However, in order to further increase the total number of sProteins, the combination of C8 cartridge enrichment with GelFree enrichment is favored because the latter complemented n = 48 more sProteins compared to the C8 cartridge approach resulting in n = 343 sProteins. Among all detected sProteins we were able to identify 79 so far uncharacterized sProteins, with no described protein evidence in the current released database. In total, 34 of those uncharacterized sProteins are localized in gene clusters conserved between different bacteria species allowing functional predictions. This study improves the assessment of sProtein detection and enables their functional characterization in future experiments.
ID 22828
dauerhafte UFZ-Verlinkung
Petruschke, H., Anders, J., Stadler, P.F., Jehmlich, N., von Bergen, M. (2020):
Enrichment and identification of small proteins in a simplified human gut microbiome
J. Proteomics 213 , art. 103604