Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1002/cssc.201903428
Lizenz creative commons licence
Titel (primär) Response‐surface‐optimized and scaled‐up microbial electrosynthesis of chiral alcohols
Autor Mayr, J.C.; Rosa, L.F.M.; Klinger, N.; Grosch, J.-H.; Harnisch, F. ORCID logo ; Spiess, A.C.
Quelle ChemSusChem
Erscheinungsjahr 2020
Department UMB
Band/Volume 13
Heft 7
Seite von 1808
Seite bis 1816
Sprache englisch
Keywords biocatalysis; cofactor regeneration; electrobiotechnology; enantioselectivity; microbial electrosynthesis
Abstract A whole variety of enzymes can be easily incorporated and overexpressed within Escherichia coli cells via plasmids, making it an ideal chassis for bioelectrosynthesis. We recently demonstrated microbial electrosynthesis (MES) of chiral alcohols using genetically modified E. coli with plasmid‐incorporated and overexpressed enzymes and methyl viologen as mediator for electron transfer. Here, this model system, using NADPH‐dependent alcohol dehydrogenase from Lactobacillus brevis to convert acetophenone to ( R )‐1‐phenylethanol, was assessed by a Design of Experiment (DoE) approach. Process optimization was achieved with a 2.4‐fold increased yield of 94±7%, a 3.9‐fold increased reaction rate of 324±67 μM h ‐1 , and a coulombic efficiency of up to 68±7%, while maintaining an excellent enantioselectivity of >99%. Subsequent scale‐up to 1 L using electrobioreactors under batch and fed‐batch conditions increased the titer of ( R )‐1‐phenylethanol to 12.8±2.0 mM and paves the way to further develop E. coli into a universal chassis for MES in a standard biotechnological process environment.
dauerhafte UFZ-Verlinkung
Mayr, J.C., Rosa, L.F.M., Klinger, N., Grosch, J.-H., Harnisch, F., Spiess, A.C. (2020):
Response‐surface‐optimized and scaled‐up microbial electrosynthesis of chiral alcohols
ChemSusChem 13 (7), 1808 - 1816 10.1002/cssc.201903428