Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Gastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss
Autor Haange, S.-B.; Jehmlich, N.; Krügel, U.; Hintschich, C.; Wehrmann, D.; Hankir, M.; Seyfried, F.; Froment, J.; Hübschmann, T.; Müller, S.; Wissenbach, D.K.; Kang, K.H.; Buettner, C.; Panagiotou, G.; Noll, M.; Rolle-Kampczyk, U.; Fenske, W.; von Bergen, M.;
Journal / Serie Microbiome
Erscheinungsjahr 2020
Department UMB; MOLSYB;
Band/Volume 8
Sprache englisch;
POF III (gesamt) F11;
Supplements https://static-content.springer.com/esm/art%3A10.1186%2Fs40168-020-0788-1/MediaObjects/40168_2020_788_MOESM1_ESM.docx
https://static-content.springer.com/esm/art%3A10.1186%2Fs40168-020-0788-1/MediaObjects/40168_2020_788_MOESM2_ESM.xlsx
Abstract Background
Roux-en-Y gastric bypass (RYGB) surgery is a last-resort treatment to induce substantial and sustained weight loss in cases of severe obesity. This anatomical rearrangement affects the intestinal microbiota, but so far, little information is available on how it interferes with microbial functionality and microbial-host interactions independently of weight loss.

Methods
A rat model was employed where the RYGB-surgery cohort is compared to sham-operated controls which were kept at a matched body weight by food restriction. We investigated the microbial taxonomy and functional activity using 16S rRNA amplicon gene sequencing, metaproteomics, and metabolomics on samples collected from theileum, the cecum, and the colon, and separately analysed the lumen and mucus-associated microbiota.

Results
Altered gut architecture in RYGB increased the relative occurrence of Actinobacteria, especially Bifidobacteriaceae and Proteobacteria, while in general, Firmicutes were decreased although Streptococcaceae and Clostridium perfringens were observed at relative higher abundances independent of weight loss. A decrease of conjugated and secondary bile acids was observed in the RYGB-gut lumen. The arginine biosynthesis pathway in the microbiota was altered, as indicated by the changes in the abundance of upstream metabolites and enzymes, resulting in lower levels of arginine and higher levels of aspartate in the colon after RYGB.
ID 22813
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22813
Haange, S.-B., Jehmlich, N., Krügel, U., Hintschich, C., Wehrmann, D., Hankir, M., Seyfried, F., Froment, J., Hübschmann, T., Müller, S., Wissenbach, D.K., Kang, K.H., Buettner, C., Panagiotou, G., Noll, M., Rolle-Kampczyk, U., Fenske, W., von Bergen, M. (2020):
Gastric bypass surgery in a rat model alters the community structure and functional composition of the intestinal microbiota independently of weight loss
Microbiome 8 , art. 13