Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00294-007-0138-x
Volltext Shareable Link
Titel (primär) Interaction with mycorrhiza helper bacterium Streptomyces sp. AcH 505 modifies organisation of actin cytoskeleton in the ectomycorrhizal fungus Amanita muscaria (fly agaric)
Autor Schrey, S.D.; Salo, V.; Raudaskoski, M.; Hampp, R.; Nehls, U.; Tarkka, M.T.
Quelle Current Genetics
Erscheinungsjahr 2007
Department BOOEK
Band/Volume 52
Heft 2
Seite von 77
Seite bis 85
Sprache englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs00294-007-0138-x/MediaObjects/294_2007_138_MOESM1_ESM.doc
https://static-content.springer.com/esm/art%3A10.1007%2Fs00294-007-0138-x/MediaObjects/294_2007_138_MOESM2_ESM.doc
Keywords Actin cytoskeleton; Hyphal tip growth; Mycorrhiza helper bacterium
Abstract The actin cytoskeleton (AC) of fungal hyphae is a major determinant of hyphal shape and morphogenesis, implicated in controlling tip structure and secretory vesicle delivery. Hyphal growth of the ectomycorrhizal fungus Amanita muscaria and symbiosis formation with spruce are promoted by the mycorrhiza helper bacterium Streptomyces sp. AcH 505 (AcH 505). To investigate structural requirements of growth promotion, the effect of AcH 505 on A. muscaria hyphal morphology, AC and actin gene expression were studied. Hyphal diameter and mycelial density decreased during dual culture (DC), and indirect immunofluorescence microscopy revealed that the dense and polarised actin cap in hyphal tips of axenic A. muscaria changes to a loosened and dispersed structure in DC. Supplementation of growth medium with cell-free bacterial supernatant confirmed that reduction in hyphal diameter and AC changes occurred at the same stage of growth. Transcript levels of both actin genes isolated from A. muscaria remained unaltered, indicating that AC changes are regulated by reorganisation of the existing actin pool. In conclusion, the AC reorganisation appears to result in altered hyphal morphology and faster apical extension. The thus improved spreading of hyphae and increased probability to encounter plant roots highlights a mechanism behind the mycorrhiza helper effect.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2274
Schrey, S.D., Salo, V., Raudaskoski, M., Hampp, R., Nehls, U., Tarkka, M.T. (2007):
Interaction with mycorrhiza helper bacterium Streptomyces sp. AcH 505 modifies organisation of actin cytoskeleton in the ectomycorrhizal fungus Amanita muscaria (fly agaric)
Curr. Genet. 52 (2), 77 - 85