Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) The simplified human intestinal microbiota (SIHUMIx) shows high structural and functional resistance against changing transit times in in vitro bioreactors
Autor Schäpe, S.S.; Krause, J.L.; Engelmann, B.; Fritz-Wallace, K.; Schattenberg, F.; Liu, Z.; Müller, S.; Jehmlich, N.; Rolle-Kampczyk, U.; Herberth, G.; von Bergen, M.;
Journal / Serie Microorganisms
Erscheinungsjahr 2019
Department UMB; IMMU; MOLSYB;
Band/Volume 7
Heft 12
Sprache englisch;
POF III (gesamt) F11; T42;
Supplements https://www.mdpi.com/2076-2607/7/12/641/s1
Keywords In vitro model; microbial community; flow cytometry; metaproteomics; metabolomics; short-chain fatty acids; intestinal microbiota; SIHUMIx; bioreactor
Abstract Many functions in host–microbiota interactions are potentially influenced by intestinal transit times, but little is known about the effects of altered transition times on the composition and functionality of gut microbiota. To analyze these effects, we cultivated the model community SIHUMIx in bioreactors in order to determine the effects of varying transit times (TT) on the community structure and function. After five days of continuous cultivation, we investigated the influence of different medium TT of 12 h, 24 h, and 48 h. For profiling the microbial community, we applied flow cytometric fingerprinting and revealed changes in the community structure of SIHUMIx during the change of TT, which were not associated with changes in species abundances. For pinpointing metabolic alterations, we applied metaproteomics and metabolomics and found, along with shortening the TT, a slight decrease in glycan biosynthesis, carbohydrate, and amino acid metabolism and, furthermore, a reduction in butyrate, methyl butyrate, isobutyrate, valerate, and isovalerate concentrations. Specifically, B. thetaiotaomicron was identified to be affected in terms of butyrate metabolism. However, communities could recover to the original state afterward. This study shows that SIHUMIx showed high structural stability when TT changed—even four-fold. Resistance values remained high, which suggests that TTs did not interfere with the structure of the community to a certain degree.
ID 22713
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22713
Schäpe, S.S., Krause, J.L., Engelmann, B., Fritz-Wallace, K., Schattenberg, F., Liu, Z., Müller, S., Jehmlich, N., Rolle-Kampczyk, U., Herberth, G., von Bergen, M. (2019):
The simplified human intestinal microbiota (SIHUMIx) shows high structural and functional resistance against changing transit times in in vitro bioreactors
Microorganisms 7 (12), art. 641