Details zur Publikation
|Titel (primär)||Quantification of glyphosate and AMPA from microbiome reactor fluids|
|Autor||Fritz-Wallace, K.; Engelmann, B.; Krause, J.L.; Schäpe, S.S.; Pöppe, J.; Herberth, G. ; Rösler, U.; Jehmlich, N.; von Bergen, M.; Rolle-Kampczyk, U.|
|Journal / Serie||Rapid Communications in Mass Spectrometry|
|Keywords||Glyphosate; AMPA; LC/MS/MS; microbiome bioreactor; Roundup|
An optimized protocol enables the analysis of both glyphosate and AMPA by simple extraction with methanol:acetonitrile:water (2:3:1) without further enrichment steps. Glyphosate and AMPA are separated by liquid chromatography on an amide column and identified and quantified with a targeted MS/MS method on a QTRAP 5500 system (AB Sciex).
Our method has a limit of detection (LOD) in extracted water samples of < 2 ng/mL for both glyphosate and AMPA. In complex intestinal medium the LOD is 2 ng/mL and 5 ng/mL for glyphosate and AMPA, respectively. These LODs allow for measurement at exposure‐relevant concentrations. Glyphosate levels in a bioreactor model of porcine colon were determined and consequently it was verified whether AMPA was produced by porcine gut microbiota.
The method presented here allows quantitation of glyphosate and AMPA in complex bioreactor fluids and thus enables studies on the impact of glyphosate and its metabolism on the intestinal microbiota. In addition, the extraction protocol is compatible with an untargeted metabolomics analysis, thus allowing other perturbations caused by glyphosate in the same sample to be looked for.
|Fritz-Wallace, K., Engelmann, B., Krause, J.L., Schäpe, S.S., Pöppe, J., Herberth, G., Rösler, U., Jehmlich, N., von Bergen, M., Rolle-Kampczyk, U. (2020):
Quantification of glyphosate and AMPA from microbiome reactor fluids
Rapid Commun. Mass Spectrom. 34 (7), e8668