Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.copbio.2019.11.001
Volltext Autorenversion
Titel (primär) Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding
Autor Dusny, C.; Grünberger, A.
Quelle Current Opinion in Biotechnology
Erscheinungsjahr 2020
Department SOMA
Band/Volume 63
Seite von 26
Seite bis 33
Sprache englisch
UFZ Querschnittsthemen MIKAT;
Abstract Our understanding of the microbial cell is based on averaged values from bulks. Microfluidic single-cell analysis holds the promise of understanding cellular processes from a single cell perspective. But what is needed to measure single-cell physiology and to disclose the consequences of individuality for biotechnology? Current single-cell research is not yet able to provide all the necessary insights, but innovative approaches now emerge that propel the field towards a better understanding of cellular processes via quantitative physiology. Here, we critically review novel single-cell technologies that enable us to control cellular input parameters such as environmental conditions and to measure intracellular processes, as well as novel approaches that enable for the first time to quantify non-averaged cell-specific rates and yields. Finally, we demonstrate how integrating microfluidic single-cell analysis into established population-based experimental workflows might unlock its full potential for biotechnology research in the future.
dauerhafte UFZ-Verlinkung
Dusny, C., Grünberger, A. (2020):
Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding
Curr. Opin. Biotechnol. 63 , 26 - 33 10.1016/j.copbio.2019.11.001