Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s11356-019-06505-z
Volltext Shareable Link
Titel (primär) Deiodination in the presence of Dehalococcoides mccartyi strain CBDB1: comparison of the native enzyme and co-factor vitamin B12
Autor El-Athman, F.; Adrian, L.; Jekel, M.; Putschew, A.
Quelle Environmental Science and Pollution Research
Erscheinungsjahr 2019
Department ISOBIO
Band/Volume 26
Heft 31
Seite von 32636
Seite bis 32644
Sprache englisch
Supplements https://static-content.springer.com/esm/art%3A10.1007%2Fs11356-019-06505-z/MediaObjects/11356_2019_6505_MOESM1_ESM.docx
Keywords Deiodination; Iodinated contrast media; Triiodobenzoic acid; Vitamin B12; Dehalococcoides mccartyi; CBDB1; Organohalide-respiration; Reductive dehalogenases
Abstract Triiodinated benzoic acid derivatives are widely used as contrast media for medical examinations and are found at high concentrations in urban aquatic environments. During bank filtration, deiodination of iodinated contrast media has been observed under anoxic/anaerobic conditions. While several bacterial strains capable of dechlorination and debromination have been isolated and characterized, deiodination has not yet been shown for an isolated strain. Here, we investigate dehalogenation of iodinated contrast media (ICM), triiodobenzoic acids (TIBA), and analogous chlorinated compounds by Dehalococcoides mccartyi strain CBDB1 and its corrinoid co-factor vitamin B12. No cell growth of CBDB1 was observed using iodinated compounds as electron acceptor. Only negligible deiodination occurred for ICM, whereas 2,3,5-TIBA was nearly completely deiodinated by CBDB1 without showing cell growth. Furthermore, TIBA inhibited growth with hexachlorobenzene which is usually a well-suited electron acceptor for strain CBDB1, indicating that TIBA is toxic for CBDB1. The involvement of CBDB1 enzymes in the deiodination of TIBA was verified by the absence of deiodination activity after heat inactivation. Adding iodopropane also inhibited the deiodination of TIBA by CBDB1 cells, indicating the involvement of a corrinoid-enzyme in the reductive TIBA deiodination. The results further suggest that the involved electron transport is decoupled from proton translocation and therefore growth.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22356
El-Athman, F., Adrian, L., Jekel, M., Putschew, A. (2019):
Deiodination in the presence of Dehalococcoides mccartyi strain CBDB1: comparison of the native enzyme and co-factor vitamin B12
Environ. Sci. Pollut. Res. 26 (31), 32636 - 32644 10.1007/s11356-019-06505-z