UFZ - Publikationsverzeichnis - Helmholtz-Zentrum für Umweltforschung UFZ

Details zur Publikation

Kategorie	Textpublikation
Referenztyp	Qualifizierungsarbeiten
Volltext	Publikationsdokument
Titel (primär)	Biocatalyst design for 1,2-propanediol synthesis from CO₂ employing the photoautotrophic strain Synechocystis sp. PCC 6803
Autor	David, C.
Quelle	PhD Dissertation
Erscheinungsjahr	2019
Department	SOMA
Band/Volume	4/2019
Seite bis	149
Sprache	englisch
Keywords	Photoautotropher Mikroorganismus; Biokatalyse; CO2; Biocatalysis; Photoautotrophic microogranism; Stoffwechsel; Metabolism
UFZ Bestand	Leipzig, Bibliothek, Reportsammlung, 00534215, 19-0322 F/E
Abstract	The application of photoautotrophic microorganisms in biotechnology enables the development of next-generation microbial cell factories, which utilize sunlight to (re-)activate carbon dioxide. This enables the cells to provide carbon skeletons for the production of chemical feedstock and biofuels without the need for an organic carbon and energy source. However, up to now, the production rates and product titers achieved with these cell factories are low and far away from an economically feasible process. Further progress has to be made in biocatalyst and process engineering to increase the understanding and fully exploit the capacities of these biocatalysts. This work focused on the development of a photoautotrophic whole-cell biocatalyst for the production of 1,2-propanediol (propylene glycol). Particular focus was placed on the interconnection between the accumulation of internal storage compounds and the influence on the production capacity of the synthetic pathway. A synthetic pathway comprising a methylglyoxal synthase, an aldehyde reductase, and an alcohol dehydrogenase was inserted into Synechocystis sp. PCC 6803 to convert dihydroxyacetone phosphate to methylglyoxal, which is subsequently reduced to acetol and then to 1,2-propanediol.
dauerhafte UFZ-Verlinkung	https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22121
David, C. (2019): Biocatalyst design for 1,2-propanediol synthesis from CO₂ employing the photoautotrophic strain Synechocystis sp. PCC 6803 Dissertation, TU Dresden PhD Dissertation 4/2019 Helmholtz-Zentrum für Umweltforschung - UFZ, Leipzig, 149 pp.