Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.molimm.2019.05.008
Titel (primär) Sequencing of VDJ genes in Lepus americanus confirms a correlation between VHn expression and the leporid species continent of origin
Autor Pinheiro, A.; de Sousa-Pereira, P.; Almeida, T.; Ferreira, C.C.; Otis, T.; Boudreau, M.R.; Seguin, J.L.; Lanning, D.K.; Esteves, P.J.
Quelle Molecular Immunology
Erscheinungsjahr 2019
Department NSF
Band/Volume 112
Seite von 182
Seite bis 187
Sprache englisch
Keywords Leporids; Lepus americanus; IGHV; Adaptive selection
Abstract Leporid VH genes used in the generation of their primary antibody repertoire exhibit highly divergent lineages. For the European rabbit (Oryctolagus cuniculus) four VHa lineages have been described, the a1, a2, a3 and a4. Hares (Lepus spp.) and cottontail (Sylvilagus floridanus) express one VHa lineage each, the a2L and the a5, respectively, along with a more ancient lineage, the Lepus spp. sL and S. floridanus sS. Both the European rabbit and the Lepus europaeus use a third lineage, VHn, in a low proportion of their VDJ rearrangements. The VHn genes are a conserved ancestral polymorphism that is being maintained in the leporid genome.Their usage in a low proportion of VDJ rearrangements by both European rabbit and L. europaeus but not S. floridanus has been argued to be a remnant of an ancient European leporid immunologic response to pathogens. To address this hypothesis, in this study we sequenced VDJ rearranged genes for another North American leporid, L. americanus. Our results show that L. americanus expressed these genes less frequently and in a highly modified fashion compared to the European Lepus species. Our results suggest that the American leporid species use a different VH repertoire than the European species which may be related with an immune adaptation to different environmental conditions, such as different pathogenic agents.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22052
Pinheiro, A., de Sousa-Pereira, P., Almeida, T., Ferreira, C.C., Otis, T., Boudreau, M.R., Seguin, J.L., Lanning, D.K., Esteves, P.J. (2019):
Sequencing of VDJ genes in Lepus americanus confirms a correlation between VHn expression and the leporid species continent of origin
Mol. Immunol. 112 , 182 - 187 10.1016/j.molimm.2019.05.008