Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1038/s41598-019-45993-4 |
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Titel (primär) | Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens |
Autor | Philipp, K.; Lemke, F.; Scholz, S.
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Quelle | Scientific Reports |
Erscheinungsjahr | 2019 |
Department | BIOTOX |
Band/Volume | 9 |
Seite von | art. 9532 |
Sprache | englisch |
Abstract | Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding. We propose a bi-actuator adaptive lens that simultaneously enables axial scanning and the correction of specimen-induced spherical aberrations with a compact setup. Using the bi-actuator lens in a confocal microscope, we show diffraction-limited axial scanning up to 340 μm deep inside a phantom specimen. The application of this technique to in vivo measurements of zebrafish embryos with reporter-gene-driven fluorescence in a thyroid gland reveals substructures of the thyroid follicles, indicating that the bi-actuator adaptive lens is a meaningful supplement to the existing adaptive optics toolset. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22023 |
Philipp, K., Lemke, F., Scholz, S., Wallrabe, U., Wapler, M.C., Koukourakis, N., Czarske, J.W. (2019): Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens Sci. Rep. 9 , art. 9532 10.1038/s41598-019-45993-4 |