Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1038/s41598-019-45993-4 |
| Lizenz |
|
| Titel (primär) | Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens |
| Autor | Philipp, K.; Lemke, F.; Scholz, S.; Wallrabe, U.; Wapler, M.C.; Koukourakis, N.; Czarske, J.W. |
| Journal / Serie | Scientific Reports |
| Erscheinungsjahr | 2019 |
| Department | BIOTOX |
| Band/Volume | 9 |
| Seite von | art. 9532 |
| Sprache | englisch |
| Abstract | Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding. We propose a bi-actuator adaptive lens that simultaneously enables axial scanning and the correction of specimen-induced spherical aberrations with a compact setup. Using the bi-actuator lens in a confocal microscope, we show diffraction-limited axial scanning up to 340 μm deep inside a phantom specimen. The application of this technique to in vivo measurements of zebrafish embryos with reporter-gene-driven fluorescence in a thyroid gland reveals substructures of the thyroid follicles, indicating that the bi-actuator adaptive lens is a meaningful supplement to the existing adaptive optics toolset. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=22023 |
| Philipp, K., Lemke, F., Scholz, S., Wallrabe, U., Wapler, M.C., Koukourakis, N., Czarske, J.W. (2019): Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens Sci. Rep. 9 , art. 9532 |
|