Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1264/jsme2.ME18134
Titel (primär) Visualization of the sorption of nickel within exopolymer microdomains of bacterial microcolonies using confocal and scanning electron microscopy
Autor Lawrence, J.R.; Swerhone, G.D.W.; Neu, T.R.
Quelle Microbes and Environments
Erscheinungsjahr 2019
Department FLOEK
Band/Volume 34
Heft 1
Seite von 73
Seite bis 82
Sprache englisch
Keywords Newport Green; SEM; microdomains; exopolymer; confocal
Abstract The sorption and distribution of nickel, a common metal contaminant in aquatic systems, were assessed in bacterial microcolonies using a combination of fluorescent staining with Newport Green and confocal laser scanning microscopy (CLSM) with confirmation by scanning electron microscopy (SEM) and X-ray microprobe analyses. CLSM with Newport Green, selected fluor-conjugated lectins, and DNA staining allowed for the discrimination of the microdomains present in the microcolony exopolymeric matrix and detection of bound nickel. This approach avoided the artefacts associated with drying and fixation required by analytical electron microscopy. The results obtained indicated that specific microcolonies within river biofilms sorbed nickel within limited microdomains present in the complex tripartite exopolymeric matrix surrounding bacterial cells. Sorption occurred such that nickel was concentrated within the exopolymeric matrix, but not directly associated with cells. These microdomains appeared to have neutral pH and be dominated by negatively charged residues favoring the sorption of nickel and other cations. These results also suggest an important role for specific community members in the sorption and concentration of metals in aquatic biofilm communities.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21736
Lawrence, J.R., Swerhone, G.D.W., Neu, T.R. (2019):
Visualization of the sorption of nickel within exopolymer microdomains of bacterial microcolonies using confocal and scanning electron microscopy
Microbes Environ. 34 (1), 73 - 82 10.1264/jsme2.ME18134