Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.3389/fbioe.2019.00060 |
Lizenz  |
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| Titel (primär) | Long-term behavior of defined mixed cultures of Geobacter sulfurreducens and Shewanella oneidensis in bioelectrochemical systems |
| Autor | Engel, C.; Schattenberg, F.; Dohnt, K.; Schröder, U.; Müller, S.; Krull, R. |
| Quelle | Frontiers in Bioengineering and Biotechnology |
| Erscheinungsjahr | 2019 |
| Department | UMB |
| Band/Volume | 7 |
| Seite von | art. 60 |
| Sprache | englisch |
| Supplements | https://ndownloader.figshare.com/files/14719100 |
| Keywords | bioelectrochemical systems, biofilm thickness, flow cytometry, Geobacter sulfurreducens, microbial electrolysis cell, mixed culture, planktonic, Shewanella oneidensis |
| Abstract | This work aims to investigate the long-term behavior of interactions of electrochemically active bacteria in bioelectrochemical systems. The electrochemical performance and biofilm characteristics of pure cultures of Geobacter sulfurreducens and Shewanella oneidensis are being compared to a defined mixed culture of both organisms. While S. oneidensis pure cultures did not form cohesive and stable biofilms on graphite anodes and only yielded 0.034 ± 0.011 mA/cm2 as maximum current density by feeding of each 5 mM lactate and acetate, G. sulfurreducens pure cultures formed 69 μm thick, area-wide biofilms with 10 mM acetate as initial substrate concentration and yielded a current of 0.39 ± 0.09 mA/cm2. Compared to the latter, a defined mixed culture of both species was able to yield 38% higher maximum current densities of 0.54 ± 0.07 mA/cm2 with each 5 mM lactate and acetate. This increase in current density was associated with a likewise increased thickness of the anodic biofilm to approximately 93 μm. It was further investigated whether a sessile incorporation of S. oneidensis into the mixed culture biofilm, which has been reported previously for short-term experiments, is long-term stable. The results demonstrate that S. oneidensis was not stably incorporated into the biofilm; rather, the planktonic presence of S. oneidensis has a positive effect on the biofilm growth of G. sulfurreducens and thus on current production. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21726 |
| Engel, C., Schattenberg, F., Dohnt, K., Schröder, U., Müller, S., Krull, R. (2019): Long-term behavior of defined mixed cultures of Geobacter sulfurreducens and Shewanella oneidensis in bioelectrochemical systems Front. Bioeng. Biotechnol. 7 , art. 60 10.3389/fbioe.2019.00060 |
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