Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Buchkapitel |
DOI | 10.1007/978-1-4939-8695-8_12 |
Titel (primär) | Sulfur-34S and 36S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses |
Titel (sekundär) | Microbial proteomics : methods and protocols |
Autor | Herbst, F.-A.; Jehmlich, N.
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Herausgeber | Becher, D. |
Quelle | Methods in Molecular Biology |
Erscheinungsjahr | 2018 |
Department | MOLSYB |
Band/Volume | 1841 |
Seite von | 163 |
Seite bis | 174 |
Sprache | englisch |
Keywords | Sulfur stable isotope labeling of amino acids for quantification; SULAQ; Sulfur-34S; Sulfur-36S; Quantitative proteomics |
Abstract | Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21394 |
Herbst, F.-A., Jehmlich, N., von Bergen, M., Schmidt, F. (2018): Sulfur-34S and 36S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses In: Becher, D. (ed.) Microbial proteomics : methods and protocols Methods in Molecular Biology 1841 Springer Nature, p. 163 - 174 10.1007/978-1-4939-8695-8_12 |