Details zur Publikation

Referenztyp Buchkapitel
DOI / URL Link
Titel (primär) Sulfur-34S and 36S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses
Titel (sekundär) Microbial proteomics : methods and protocols
Autor Herbst, F.-A.; Jehmlich, N.; von Bergen, M.; Schmidt, F.;
Herausgeber Becher, D.;
Journal / Serie Methods in Molecular Biology
Erscheinungsjahr 2018
Department MOLSYB;
Band/Volume 1841
Sprache englisch;
POF III (gesamt) T41;
Keywords Sulfur stable isotope labeling of amino acids for quantification; SULAQ; Sulfur-34S; Sulfur-36S; Quantitative proteomics
Abstract

Quantitative proteome profiling of microorganisms by isotopic labeling of amino acids is still a challenge, because only microorganisms with auxotrophic character are able to embed amino acids into their biomass in a quantitatively correct manner. Here, we describe an isotopic labeling technique (sulfur stable isotope labeling of amino acids for quantification, SULAQ) for the sulfur-containing amino acids cysteine and methionine in a broad range of organisms. The metabolic labeling approach is suitable for gel-based and gel-free protein analysis.

ID 21394
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21394
Herbst, F.-A., Jehmlich, N., von Bergen, M., Schmidt, F. (2018):
Sulfur-34S and 36S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses
In: Becher, D. (ed.)
Microbial proteomics : methods and protocols
Methods in Molecular Biology 1841
Humana Press, p. 163 - 174