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Titel (primär) Application of next‐generation sequencing technologies to conservation of wood‐inhabiting fungi
Autor Purahong, W.; Wubet, T. ORCID logo ; Krüger, D.; Buscot, F.
Journal / Serie Conservation Biology
Erscheinungsjahr 2019
Department BZF; BOOEK; iDiv
Band/Volume 33
Heft 3
Seite von 716
Seite bis 724
Sprache englisch
Keywords Conservation strategy; Deadwood; Deadwood enrichment; Forest management; ITS; Microbial conservation; Next‐generation sequencing
Abstract Next‐generation sequencing (NGS) has significantly increased knowledge of microbial communities and their distribution. However, it is still not common to apply NGS technology to the field of microbial conservation. This study aims to use NGS technologies to evaluate conservation strategies for wood‐inhabiting fungi. Evaluating a deadwood experiment three years after it was established, we specifically examine which species and species combinations of trees promote the highest richness of wood‐inhabiting fungi. Our results show that deadwood enrichment is an effective strategy and using logs of six tree species, either those with the highest wood‐inhabiting fungal α and γ diversity or those with the highest β diversity, we maintain >1000 OTUs spread over a wide range of taxonomic groups. In comparison, a conservation strategy based only on the results of sporocarp surveys yielded 591 OTUs. This result highlights the need to use NGS approaches to inform microbial conservation strategies. We also identified a conservation strategy that simultaneously conserves wood‐inhabiting fungi and saproxylic beetles. Apart from deadwood volume, we suggest that the data on deadwood quality and species are also among the relevant indicators, especially for WIF diversity, thus they should be urgently incorporated in the forest assessment and monitoring systems in Central Europe.
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Purahong, W., Wubet, T., Krüger, D., Buscot, F. (2019):
Application of next‐generation sequencing technologies to conservation of wood‐inhabiting fungi
Conserv. Biol. 33 (3), 716 - 724