Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Application of experimental polystyrene partition constants and diffusion coefficients to predict the sorption of neutral organic chemicals to multiwell plates in in vivo and in vitro bioassays
Autor Fischer, F.C.; Cirpka, O.A.; Goss, K.-U.; Henneberger, L.; Escher, B.I.;
Journal / Serie Environmental Science & Technology
Erscheinungsjahr 2018
Department AUC; ZELLTOX;
Band/Volume 52
Heft 22
Sprache englisch;
POF III (gesamt) T42;
Supplements https://pubs.acs.org/doi/suppl/10.1021/acs.est.8b04246/suppl_file/es8b04246_si_003.pdf
https://pubs.acs.org/doi/suppl/10.1021/acs.est.8b04246/suppl_file/es8b04246_si_004.xlsx
Abstract Sorption to the polystyrene (PS) of multiwell plates can affect the exposure to organic chemicals over time in in vitro and in vivo bioassays. Experimentally determined diffusion coefficients in PS (DPS) were in a narrow range of 1.25 to 8.0 · 10–16 m2 s–1 and PS-water partition constants (KPS/w) ranged from 0.04 to 5.10 log-units for 22 neutral organic chemicals. A kinetic model, which explicitly accounts for diffusion in the plastic, was applied to predict the depletion of neutral organic chemicals from different bioassay media by sorption to various multiwell plate formats. For chemicals with log Kow > 3, the medium concentrations decreased rapidly and considerably in the fish embryo toxicity assay but medium concentrations remained relatively constant in the cell-based bioassays with medium containing 10% fetal bovine serum (FBS), emphasizing the ability of the protein- and lipid-rich medium to compensate for losses by multiwell plate sorption. The PS sorption data may serve not only for exposure assessment in bioassays but also to model the contaminant uptake by and release from plastic packaging material and the chemical transport by PS particles in the environment.
ID 21223
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=21223
Fischer, F.C., Cirpka, O.A., Goss, K.-U., Henneberger, L., Escher, B.I. (2018):
Application of experimental polystyrene partition constants and diffusion coefficients to predict the sorption of neutral organic chemicals to multiwell plates in in vivo and in vitro bioassays
Environ. Sci. Technol. 52 (22), 13511 - 13522